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PMID: 2872165 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Filamentous hemagglutinin has a major role in mediating adherence of Bordetella pertussis to human WiDr cells.

Infection and immunity ·Vol. 52 ·No. 3 ·1986-06-00 ·Pages 695-701

Urisu A, Cowell JL, Manclark CR

Abstract

[35S]methionine-labeled Bordetella pertussis adhered to monolayers of WiDr cells, an epitheliumlike cell line from a human intestinal carcinoma. Adherence was proportional to the density of the WiDr cells and to the concentration of B. pertussis in the assay. Adherence of virulent phase I strains Tohama phase I, 114, and BP338 was much greater than adherence of avirulent strains Tohama phase III and 423 phase IV. Mutants deficient in the production of the filamentous hemagglutinin (FHA) were hemagglutination negative and adhered to WiDr cells much less efficiently than the parent strains. Preincubation of B. pertussis cells with FHA increased their hemagglutination activity and adherence to WiDr cells. Goat antibody to FHA inhibited, in a dose-dependent manner, the adherence of strain Tohama I but not the adherence of FHA-deficient mutant Tohama 325. At similar protein concentrations, normal goat antibody, goat antibody to pertussis toxin, or the Fab fragments of goat antibody to serotype 2 fimbriae had no effect on adherence. Also, an FHA-positive strain without fimbriae showed high adherence, while a fimbriated FHA-deficient mutant adhered poorly. Our data indicate that FHA plays a major role in adherence of B. pertussis to human WiDr cells. Fimbriae do not appear to mediate attachment of B. pertussis to WiDr cells.

MeSH Terms
Adhesiveness Antibodies, Bacterial/immunology Antigen-Antibody Reactions Bacterial Proteins/immunology,metabolism Bordetella pertussis/pathogenicity,physiology Cells, Cultured Epithelium/microbiology Fimbriae, Bacterial/immunology,metabolism Hemagglutinins/immunology,metabolism Humans
Chemicals
Antibodies, Bacterial Bacterial Proteins Hemagglutinins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Urisu A
Cowell J L
Manclark C R
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29 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.52.3.695-701.1986
Published
1986-06-00
Pages
695-701
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260913
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.52.3.695-701.1986 PMC Full Text View journal details
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