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PMID: 2892128 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A highly evolutionarily conserved mitochondrial protein is structurally related to the protein encoded by the Escherichia coli groEL gene.

Molecular and cellular biology ·Vol. 8 ·No. 1 ·1988-01-00 ·Pages 371-80

McMullin TW, Hallberg RL

Abstract

We recently reported that a Tetrahymena thermophila 58-kilodalton (kDa) mitochondrial protein (hsp58) was selectively synthesized during heat shock. In this study, we show that hsp58 displayed antigenic similarity with mitochondrially associated proteins from Saccharomyces cerevisiae (64 kDa), Xenopus laevis (60 kDa), Zea mays (62 kDa), and human cells (59 kDa). Furthermore, a 58-kDa protein from Escherichia coli also exhibited antigenic cross-reactivity to an antiserum directed against the T. thermophila mitochondrial protein. The proteins from S. cerevisiae and E. coli antigenically related to hsp58 were studied in detail and found to share several other characteristics with hsp58, including heat inducibility and the property of associating into distinct oligomeric complexes. The T. thermophila, S. cerevisiae, and E. coli macromolecular complexes containing these related proteins had similar sedimentation characteristics and virtually identical morphologies as seen with the electron microscope. The distinctive properties of the E. coli homolog to T. thermophila hsp58 indicate that it is most likely the product of the groEL gene.

MeSH Terms
Animals Bacterial Proteins/genetics,immunology Biological Evolution Chaperonin 60 Cross Reactions DNA, Mitochondrial/genetics Escherichia coli/genetics Genes Genes, Bacterial Heat-Shock Proteins/genetics,immunology Humans Immunologic Techniques Saccharomyces cerevisiae/genetics Xenopus laevis/genetics Zea mays/genetics
Chemicals
Bacterial Proteins Chaperonin 60 DNA, Mitochondrial Heat-Shock Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McMullin T W
Zoology Department, Iowa State University, Ames 50011.
Hallberg R L
References (46)
46 references, click to expand
  1. An enzyme that removes clathrin coats: purification of an uncoating ATPase.
    J Cell Biol. 1984 Aug;99(2):723-33 PMID: 6146630
  2. hsp70: nuclear concentration during environmental stress and cytoplasmic storage during recovery.
    Cell. 1984 Mar;36(3):655-62 PMID: 6421488
  3. Quantitation and intracellular localization of the 85K heat shock protein by using monoclonal and polyclonal antibodies.
    Mol Cell Biol. 1984 Dec;4(12):2802-10 PMID: 6396506
  4. Levels of major proteins of Escherichia coli during growth at different temperatures.
    J Bacteriol. 1979 Jul;139(1):185-94 PMID: 156716
  5. Sequence of three copies of the gene for the major Drosophila heat shock induced protein and their flanking regions.
    Cell. 1980 Oct;21(3):669-79 PMID: 6777045
  6. Transient rates of synthesis of individual polypeptides in E. coli following temperature shifts.
    Cell. 1978 Mar;13(3):427-34 PMID: 350413
  7. Positive regulatory gene for temperature-controlled proteins in Escherichia coli.
    Biochem Biophys Res Commun. 1981 May 29;100(2):894-900 PMID: 7023474
  8. A nuclear mutation that post-transcriptionally blocks accumulation of a yeast mitochondrial gene product can be suppressed by a mitochondrial gene rearrangement.
    J Mol Biol. 1984 Jun 5;175(4):431-52 PMID: 6330366
  9. Efficient isolation of genes by using antibody probes.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
  10. Differential regulation of the 70K heat shock gene and related genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Aug;4(8):1454-9 PMID: 6436685
  11. Starved Tetrahymena thermophila cells that are unable to mount an effective heat shock response selectively degrade their rRNA.
    Mol Cell Biol. 1984 Oct;4(10):2170-9 PMID: 6504043
  12. Heat-shock proteins are associated with hnRNA in Drosophila melanogaster tissue culture cells.
    EMBO J. 1983;2(5):705-10 PMID: 16453451
  13. Purification and properties of groE, a host protein involved in bacteriophage assembly.
    J Mol Biol. 1979 Apr 15;129(3):375-92 PMID: 379350
  14. Rapid purification of mammalian 70,000-dalton stress proteins: affinity of the proteins for nucleotides.
    Mol Cell Biol. 1985 Jun;5(6):1229-37 PMID: 4033650
  15. Interspecific nucleotide sequence comparisons used to identify regulatory and structural features of the Drosophila hsp82 gene.
    J Mol Biol. 1986 Apr 20;188(4):499-515 PMID: 2426456
  16. Nuclear and nucleolar localization of the 72,000-dalton heat shock protein in heat-shocked mammalian cells.
    J Biol Chem. 1984 Apr 10;259(7):4501-13 PMID: 6368558
  17. Host participation in bacteriophage lambda head assembly.
    J Mol Biol. 1973 May 5;76(1):45-60 PMID: 4578100
  18. An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein.
    Cell. 1986 Jul 18;46(2):291-300 PMID: 3087629
  19. Silver staining of proteins in polyacrylamide gels.
    Anal Biochem. 1981 Nov 15;118(1):197-203 PMID: 6175245
  20. Genetic control of heat-shock protein synthesis and its bearing on growth and thermal resistance in Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1982 Feb;79(3):860-4 PMID: 7038687
  21. Effect of heat shock on ribosome structure: appearance of a new ribosome-associated protein.
    Mol Cell Biol. 1986 Jul;6(7):2527-35 PMID: 3537722
  22. Abortive bacteriophage T4 head assembly in mutants of Escherichia coli.
    J Mol Biol. 1973 May 5;76(1):61-87 PMID: 4578101
  23. Role of the host in virus assembly: cloning of the Escherichia coli groE gene and identification of its protein product.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):136-9 PMID: 343102
  24. Properties of a mutant of Escherichia coli defective in bacteriophage lambda head formation (groE). II. The propagation of phage lambda.
    J Mol Biol. 1973 May 5;76(1):25-44 PMID: 4578099
  25. Expression of Drosophila heat-shock cognate genes during heat shock and development.
    Dev Biol. 1983 Oct;99(2):418-26 PMID: 6311649
  26. Mitochondrial DNA in Xenopus laevis oocytes. I. Displacement loop occurrence.
    Dev Biol. 1974 Jun;38(2):346-55 PMID: 4857493
  27. A dot-immunobinding assay for monoclonal and other antibodies.
    Anal Biochem. 1982 Jan 1;119(1):142-7 PMID: 7072935
  28. The heat-shock response.
    Annu Rev Biochem. 1986;55:1151-91 PMID: 2427013
  29. Saccharomyces cerevisiae contains a complex multigene family related to the major heat shock-inducible gene of Drosophila.
    Mol Cell Biol. 1982 Nov;2(11):1388-98 PMID: 6761581
  30. Uncoating ATPase is a member of the 70 kilodalton family of stress proteins.
    Cell. 1986 Apr 11;45(1):3-13 PMID: 2937542
  31. Localization of mitochondria in living cells with rhodamine 123.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):990-4 PMID: 6965798
  32. Conserved features of eukaryotic hsp70 genes revealed by comparison with the nucleotide sequence of human hsp70.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6455-9 PMID: 3931075
  33. Complete sequence of the heat shock-inducible HSP90 gene of Saccharomyces cerevisiae.
    J Biol Chem. 1984 May 10;259(9):5745-51 PMID: 6325446
  34. Speculations on the functions of the major heat shock and glucose-regulated proteins.
    Cell. 1986 Sep 26;46(7):959-61 PMID: 2944601
  35. The heat shock response.
    CRC Crit Rev Biochem. 1985;18(3):239-80 PMID: 2412760
  36. Bacterial mutants which block phage assembly.
    J Supramol Struct. 1974;2(2-4):349-59 PMID: 4612252
  37. Induction of acquired thermotolerance in Tetrahymena thermophila: effects of protein synthesis inhibitors.
    Mol Cell Biol. 1985 Aug;5(8):2061-9 PMID: 3837855
  38. Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous.
    Proc Natl Acad Sci U S A. 1984 Feb;81(3):848-52 PMID: 6322174
  39. Import of an incompletely folded precursor protein into isolated mitochondria requires an energized inner membrane, but no added ATP.
    EMBO J. 1987 Aug;6(8):2449-56 PMID: 2822394
  40. Antibodies to two major chicken heat shock proteins cross-react with similar proteins in widely divergent species.
    Mol Cell Biol. 1982 Mar;2(3):267-74 PMID: 7110134
  41. Hsp70 accelerates the recovery of nucleolar morphology after heat shock.
    EMBO J. 1984 Dec 20;3(13):3095-100 PMID: 6441707
  42. A normal mitochondrial protein is selectively synthesized and accumulated during heat shock in Tetrahymena thermophila.
    Mol Cell Biol. 1987 Dec;7(12):4414-23 PMID: 3325824
  43. Xenopus hsp 70 genes are constitutively expressed in injected oocytes.
    EMBO J. 1984 Nov;3(11):2477-83 PMID: 6510409
  44. Identification of a host protein necessary for bacteriophage morphogenesis (the groE gene product).
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):131-5 PMID: 343101
  45. Expression and localization of Drosophila melanogaster hsp70 cognate proteins.
    Mol Cell Biol. 1986 Apr;6(4):1187-203 PMID: 2431275
  46. Eukaryotic Mr 83,000 heat shock protein has a homologue in Escherichia coli.
    Proc Natl Acad Sci U S A. 1987 Aug;84(15):5177-81 PMID: 3299380
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.1.371-380.1988
Published
1988-01-00
Pages
371-80
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363133
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.8.1.371-380.1988 PMC Full Text View journal details
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