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PMID: 2900832 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and expression of a Salmonella enteritidis fimbrin gene in Escherichia coli.

Journal of bacteriology ·Vol. 170 ·No. 9 ·1988-09-00 ·Pages 4216-22

Feutrier J, Kay WW, Trust TJ

Abstract

A gene bank of DNA from a human isolate of Salmonella enteritidis was constructed in the cosmid pHC79 in Escherichia coli HB101. Five clones containing 35- to 45-kilobase inserts of S. enteritidis DNA reacted in colony immunoblot assays with a polyclonal antiserum prepared against purified S. enteritidis fimbriae. Electron microscopy showed that none of the five fimbrin-producing clones produced fimbriae, yet radioimmunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis located the 14,400-molecular-weight S. enteritidis in the outer membrane fraction of three of the clones and in the periplasmic fraction of all five clones. By using an oligonucleotide probe homologous to the 5' region of the fimbrin structural gene, the fimbrin gene was located on a 5.3-kilobase HindIII fragment. In vitro transcription-translation analysis verified that this HindIII fragment subcloned into plasmid pTZ18R produced unprocessed S. enteritidis fimbrin of molecular weight 16,400. Dot blot hybridization against a selection of strains of the family Enterobacteriaceae indicated a limited distribution of the S. enteritidis fimbrin gene.

MeSH Terms
Autoradiography Cloning, Molecular Cosmids DNA Restriction Enzymes DNA, Bacterial/genetics Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Fimbriae, Bacterial/physiology Gene Expression Regulation Genes Genes, Bacterial Humans Immunoassay Membrane Glycoproteins/analysis,genetics Microfilament Proteins Nucleic Acid Hybridization Salmonella enteritidis/analysis,genetics,ultrastructure
Chemicals
DNA, Bacterial Membrane Glycoproteins Microfilament Proteins plastin DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Feutrier J
Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada.
Kay W W
Trust T J
References (34)
34 references, click to expand
  1. Role of type 1 fimbriae in the pathogenesis of ascending urinary tract infection induced by escherichia coli in mice.
    Infect Immun. 1983 Mar;39(3):1307-15 PMID: 6132876
  2. Fimbriae and infectivity in Salmonella typhimurium.
    J Med Microbiol. 1976 Nov;9(4):459-73 PMID: 794477
  3. Identification and characterization of genes determining receptor binding and pilus length of Escherichia coli type 1 pili.
    J Bacteriol. 1987 Feb;169(2):640-5 PMID: 2879830
  4. Use of a cDNA clone for the fourth component of human complement (C4) for analysis of a genetic deficiency of C4 in guinea pig.
    Proc Natl Acad Sci U S A. 1983 Sep;80(17):5387-91 PMID: 6577433
  5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  6. Construction and comparison of recombinant plasmids encoding type 1 fimbriae of members of the family Enterobacteriaceae.
    Infect Immun. 1985 May;48(2):275-9 PMID: 2580786
  7. Pilus genes of Neisseria gonorrheae: chromosomal organization and DNA sequence.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):6110-4 PMID: 6148752
  8. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate.
    J Bacteriol. 1973 Sep;115(3):717-22 PMID: 4580564
  9. A small cosmid for efficient cloning of large DNA fragments.
    Gene. 1980 Nov;11(3-4):291-8 PMID: 6260575
  10. Genes of pyelonephritogenic E. coli required for digalactoside-specific agglutination of human cells.
    EMBO J. 1984 May;3(5):1167-73 PMID: 6145590
  11. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8 PMID: 3156376
  12. Nucleotide sequences of the genes encoding type 1 fimbrial subunits of Klebsiella pneumoniae and Salmonella typhimurium.
    J Bacteriol. 1987 Dec;169(12):5831-4 PMID: 2890624
  13. Cloning and sequencing of a Moraxella bovis pilin gene.
    J Bacteriol. 1985 Jul;163(1):132-9 PMID: 2861194
  14. Frequency of gene sequences necessary for pyelonephritis-associated pili expression among isolates of Enterobacteriaceae from human extraintestinal infections.
    Infect Immun. 1984 Mar;43(3):1064-7 PMID: 6141999
  15. Organization of K88ac-encoded polypeptides in the Escherichia coli cell envelope: use of minicells and outer membrane protein mutants for studying assembly of pili.
    J Bacteriol. 1983 Jan;153(1):364-70 PMID: 6129239
  16. The fimA gene encoding the type-1 fimbrial subunit of Escherichia coli. Nucleotide sequence and primary structure of the protein.
    Eur J Biochem. 1984 Sep 3;143(2):395-9 PMID: 6147250
  17. Preparation of iodine-131 labelled human growth hormone of high specific activity.
    Nature. 1962 May 5;194:495-6 PMID: 14450081
  18. Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor.
    Infect Immun. 1977 Nov;18(2):330-7 PMID: 336541
  19. Frequency among Enterobacteriaceae of the DNA sequences encoding type 1 pili.
    J Bacteriol. 1985 May;162(2):799-803 PMID: 2859271
  20. Expression of the Pseudomonas aeruginosa PAK pilin gene in Escherichia coli.
    J Bacteriol. 1986 Feb;165(2):625-30 PMID: 2867992
  21. Localization of the receptor-binding protein adhesin at the tip of the bacterial pilus.
    Nature. 1987 Jul 2-8;328(6125):84-7 PMID: 2885755
  22. Construction and expression of recombinant plasmids encoding type 1 fimbriae of a urinary Klebsiella pneumoniae isolate.
    Infect Immun. 1983 Mar;39(3):1122-7 PMID: 6132874
  23. Cloning and expression in Escherichia coli of the gene encoding the structural subunit of Bacteroides nodosus fimbriae.
    J Bacteriol. 1984 Nov;160(2):748-54 PMID: 6150025
  24. Nucleotide sequence of pilA, the gene encoding the structural component of type 1 pili in Escherichia coli.
    J Bacteriol. 1985 Apr;162(1):454-7 PMID: 2858471
  25. Prevention of colonization of the urinary tract of mice with Escherichia coli by blocking of bacterial adherence with methyl alpha-D-mannopyranoside.
    J Infect Dis. 1979 Mar;139(3):329-32 PMID: 376757
  26. Morphogenetic expression of Bacteroides nodosus fimbriae in Pseudomonas aeruginosa.
    J Bacteriol. 1987 Jan;169(1):33-41 PMID: 2878919
  27. Purification and characterization of fimbriae from Salmonella enteritidis.
    J Bacteriol. 1986 Oct;168(1):221-7 PMID: 2875990
  28. Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate.
    Infect Immun. 1981 Sep;33(3):933-8 PMID: 6116675
  29. Cloning and expression of the pilin gene of Pseudomonas aeruginosa PAK in Escherichia coli.
    J Bacteriol. 1986 Feb;165(2):367-72 PMID: 2867989
  30. Characterization of genes encoding type 1 fimbriae of Klebsiella pneumoniae, Salmonella typhimurium, and Serratia marcescens.
    Infect Immun. 1987 Feb;55(2):281-7 PMID: 2879791
  31. The genetic determinant of adhesive function in type 1 fimbriae of Escherichia coli is distinct from the gene encoding the fimbrial subunit.
    J Bacteriol. 1986 Mar;165(3):1033-6 PMID: 2419305
  32. Type 1C fimbriae of a uropathogenic Escherichia coli strain: cloning and characterization of the genes involved in the expression of the 1C antigen and nucleotide sequence of the subunit gene.
    Gene. 1985;34(2-3):187-96 PMID: 2861144
  33. Complementation analyses of recombinant plasmids encoding type 1 fimbriae of members of the family Enterobacteriaceae.
    Infect Immun. 1985 Oct;50(1):338-40 PMID: 2864314
  34. Characterization of type 1 pili of Salmonella typhimurium LT2.
    J Bacteriol. 1980 Nov;144(2):800-5 PMID: 6107289
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.9.4216-4222.1988
Published
1988-09-00
Pages
4216-22
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211430
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.170.9.4216-4222.1988 PMC Full Text View journal details
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