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PMID: 2904678 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of a Xenopus laevis C protein cDNA: structure and expression of a heterogeneous nuclear ribonucleoprotein core protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 24 ·1988-12-00 ·Pages 9669-73

Preugschat F, Wold B

Abstract

The C proteins are major components of heterogeneous nuclear ribonucleoprotein complexes in nuclei of vertebrate cells. To begin to describe their structure, expression, and function we isolated and determined the DNA sequence of Xenopus laevis C protein cDNA clones. The protein predicted from the DNA sequence has a molecular mass of 30,916 kDa and is very similar to its human counterpart. Although mammalian genomes contain many copies of C protein sequence, the Xenopus genome contains few copies. When C protein RNA was synthesized in vitro and microinjected into stage-VI Xenopus oocytes, newly synthesized C proteins were efficiently localized in the nucleus. In vitro rabbit reticulocyte lysate and in vivo Xenopus oocyte translation systems both produce from a single mRNA two discrete polypeptide species that accumulate in a ratio similar to that of mammalian C1 and C2 proteins in vivo.

MeSH Terms
Animals Base Sequence Carrier Proteins/genetics DNA/analysis,isolation & purification Heterogeneous-Nuclear Ribonucleoprotein Group C Heterogeneous-Nuclear Ribonucleoproteins Molecular Sequence Data Molecular Weight RNA, Messenger/analysis Rabbits Ribonucleoproteins/genetics,metabolism Xenopus laevis
Chemicals
C1 HNRNP Carrier Proteins HNRNPC protein, human Heterogeneous-Nuclear Ribonucleoprotein Group C Heterogeneous-Nuclear Ribonucleoproteins RNA, Messenger Ribonucleoproteins citrate-binding transport protein DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Preugschat F
Division of Biology, California Institute of Technology, Pasadena 91125.
Wold B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.24.9669
Published
1988-12-00
Pages
9669-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282832
Subset
IM
Databases
GENBANK
J03831
External Links
PubMed source DOI: 10.1073/pnas.85.24.9669 PMC Full Text View journal details
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