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PMID: 291071 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Selection of variant neuroblastoma clones with missing or altered sodium channels.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 8 ·1979-08-00 ·Pages 4136-40

West GJ, Catterall WA

Abstract

Neurotoxins that cause persistent activation of voltage-sensitive sodium channels are highly cytotoxic to electrically excitable neuroblastoma cells. These toxins were used as selective agents to isolate variant neuroblastoma clones with missing or altered sodium channels. Of ten resistant clones analyzed, seven lacked functional sodium channels and one had a specific 40-fold increase Kd for scorpion toxin and altered voltage dependence of scorpion toxin binding. The phenotypes of these cell clones were stable for more than 100 generations, indicating that they were the result of stable genetic change.

MeSH Terms
Animals Batrachotoxins/pharmacology Clone Cells/drug effects Drug Resistance Ion Channels/drug effects Mice Neoplasms, Experimental/genetics Neuroblastoma/genetics Neurotoxins/pharmacology Scorpion Venoms/pharmacology Selection, Genetic Tetrodotoxin/pharmacology Veratridine/pharmacology
Chemicals
Batrachotoxins Ion Channels Neurotoxins Scorpion Venoms Tetrodotoxin Veratridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
West G J
Catterall W A
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.8.4136
Published
1979-08-00
Pages
4136-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383993
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.8.4136 PMC Full Text View journal details
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