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PMID: 2932798 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Control of directionality in lambda site specific recombination.

Science (New York, N.Y.) ·Vol. 230 ·No. 4728 ·1985-11-22 ·Pages 906-11

Bushman W, Thompson JF, Vargas L, Landy A

Abstract

The simple relation between the substrates and products of site-specific recombination raises questions about the control of directionality often observed in this class of DNA transactions. For bacteriophage lambda, viral integration and excision proceed by discrete pathways, and DNA substrates with the intrinsic property of recombining in only one direction can be constructed. These pathways display an asymmetric reliance on a complex array of protein binding sites, and they respond differently to changes in the concentrations of the relevant proteins. The Escherichia coli protein integration host factor (IHF) differentially affects integrative and excisive recombination, thereby influencing directionality. A four- to eightfold increase in intracellular IHF coincides with the transition from exponential to stationary phase; this provides a mechanism for growth phase-dependent regulation of recombination that makes the cellular physiology an intrinsic part of the recombination reaction.

MeSH Terms
Bacterial Proteins/genetics Bacteriophage lambda/genetics Base Sequence DNA, Bacterial/genetics DNA, Viral/genetics DNA-Binding Proteins/genetics Escherichia coli/genetics Integration Host Factors Lysogeny Recombination, Genetic Viral Proteins/genetics
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Viral DNA-Binding Proteins Integration Host Factors Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bushman W
Thompson J F
Vargas L
Landy A
References (37)
37 references, click to expand
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.2932798
Published
1985-11-22
Pages
906-11
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC1978455
Subset
IM
Grants
NIGMS NIH HHS · R01 GM033928 · United States
NIGMS NIH HHS · R01 GM062723 · United States
NIAID NIH HHS · AI13544 · United States
Databases
GENBANK
M12458, M12459
External Links
PubMed source DOI: 10.1126/science.2932798 PMC Full Text View journal details
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