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PMID: 2944114 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

U1, U2, and U6 small nuclear ribonucleoproteins (snRNPs) are associated with large nuclear RNP particles containing transcripts of an amplified gene in vivo.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 18 ·1986-09-00 ·Pages 6721-5

Sperling R, Spann P, Offen D, Sperling J

Abstract

Nuclear ribonucleoprotein (RNP) complexes that contain intact transcripts of the amplified gene for CAD, the multifunctional protein that initiates UMP synthesis in Syrian hamster cells, have been released from nuclei of Syrian hamster cells as large particulate structures that sediment at the 200S region in a sucrose gradient. By the technique of RNA hybridization, we have shown that U1, U2, and U6 small nuclear RNAs (snRNAs) cosediment with the large RNP particles in the sucrose gradients. Autoimmune sera from systemic lupus erythematosus and mixed connective tissue disease patients, characterized as anti-(U1)RNP, have further been shown to immunoprecipitate CAD RNA along with U1 and U2 snRNAs from the fractionated nuclear 200S RNP particles. We conclude that U1, U2, and U6 snRNPs are integral constituents of the 200S RNP particles. The requirement of snRNPs for RNA processing that evidently occurs on RNP particles has been recently demonstrated. Our results thus suggest that the 200S RNPs are structurally and functionally close to the native particles on which RNA processing occurs.

MeSH Terms
Aspartate Carbamoyltransferase Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) Dihydroorotase Gene Amplification Humans Multienzyme Complexes Neoplasm Proteins/genetics RNA, Small Nuclear/analysis Radioimmunoassay Ribonucleoproteins/analysis,immunology Ribonucleoproteins, Small Nuclear Transcription, Genetic
Chemicals
CAD trifunctional enzyme Multienzyme Complexes Neoplasm Proteins RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear Aspartate Carbamoyltransferase Dihydroorotase Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sperling R
Spann P
Offen D
Sperling J
References (47)
47 references, click to expand
  1. Structural organization of nuclear complexes containing DNA-like RNA.
    J Mol Biol. 1968 Apr 14;33(1):251-63 PMID: 5646646
  2. Abundant nuclear ribonucleoprotein form of CAD RNA.
    Mol Cell Biol. 1985 Mar;5(3):569-75 PMID: 3990684
  3. Visualization of RNA synthesis on chromosomes.
    Int Rev Cytol. 1972;33:1-25 PMID: 4562602
  4. N-(phosphonacetyl)-L-aspartate, a potent transition state analog inhibitor of aspartate transcarbamylase, blocks proliferation of mammalian cells in culture.
    J Biol Chem. 1974 Nov 10;249(21):6945-50 PMID: 4371054
  5. Identification of antibodies to nuclear acidic antigens by counterimmunoelectrophoresis.
    Arthritis Rheum. 1976 May-Jun;19(3):574-80 PMID: 1084750
  6. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  7. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  8. The packaging proteins of core hnRNP particles and the maintenance of proliferative cell states.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:885-98 PMID: 277323
  9. Substructure of nuclear ribonucleoprotein complexes.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:899-909 PMID: 277324
  10. Gene amplification causes overproduction of the first three enzymes of UMP synthesis in N-(phosphonacetyl)-L-aspartate-resistant hamster cells.
    J Biol Chem. 1979 Sep 10;254(17):8679-89 PMID: 381311
  11. Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3116-20 PMID: 91164
  12. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  13. Are snRNPs involved in splicing?
    Nature. 1980 Jan 10;283(5743):220-4 PMID: 7350545
  14. Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5495-9 PMID: 316537
  15. A mechanism for RNA splicing.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1877-9 PMID: 6246511
  16. Messenger RNA processing and nuclear structure: isolation of nuclear ribonucleoprotein particles containing beta-globin messenger RNA precursors.
    J Cell Biol. 1980 Oct;87(1):47-54 PMID: 6932401
  17. A small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1371-5 PMID: 6940164
  18. Subnuclear particles containing a small nuclear RNA and heterogeneous nuclear RNA.
    J Mol Biol. 1981 Jan 25;145(3):501-23 PMID: 6167725
  19. Snurps and scyrps.
    Cell. 1981 Aug;25(2):298-300 PMID: 6169438
  20. Novel models for RNA splicing that involve a small nuclear RNA.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4471-4 PMID: 6170064
  21. Methods for binding cells to plastic: application to solid phase immunoassays for cell-surface antigens.
    Methods Enzymol. 1981;73(Pt B):406-18 PMID: 7300685
  22. Use of protein A-bearing staphylococci for the immunoprecipitation and isolation of antigens from cells.
    Methods Enzymol. 1981;73(Pt B):442-59 PMID: 7029205
  23. Base-pairing interactions between small nuclear RNAs and nuclear RNA precursors as revealed by psoralen cross-linking in vivo.
    Cell. 1981 Nov;26(3 Pt 1):363-70 PMID: 6173132
  24. Small nuclear RNA U2 is base-paired to heterogeneous nuclear RNA.
    Science. 1982 Jul 30;217(4558):456-8 PMID: 6178162
  25. Transcription of a gene for human U1 small nuclear RNA.
    Cell. 1982 May;29(1):265-74 PMID: 6179630
  26. Structure of the gene for CAD, the multifunctional protein that initiates UMP synthesis in Syrian hamster cells.
    Mol Cell Biol. 1982 Mar;2(3):293-301 PMID: 6125880
  27. SnRNAs, SnRNPs, and RNA processing.
    Annu Rev Biochem. 1982;51:617-54 PMID: 6180681
  28. Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter.
    Anal Biochem. 1982 Aug;124(2):396-405 PMID: 7149237
  29. Isolation of small nuclear ribonucleoproteins containing U1, U2, U4, U5, and U6 RNAs.
    J Biol Chem. 1983 Feb 25;258(4):2604-13 PMID: 6185498
  30. Fractionation and characterization of human small nuclear ribonucleoproteins containing U1 and U2 RNAs.
    J Biol Chem. 1983 Jun 10;258(11):7181-9 PMID: 6189835
  31. Genes and pseudogenes for mouse U1 and U2 small nuclear RNAs.
    J Biol Chem. 1983 Jul 10;258(13):8151-5 PMID: 6190807
  32. The "spliceosome": yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction.
    Science. 1985 May 24;228(4702):963-7 PMID: 3890181
  33. A multicomponent complex is involved in the splicing of messenger RNA precursors.
    Cell. 1985 Aug;42(1):345-53 PMID: 3160482
  34. Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences.
    Cell. 1985 Aug;42(1):355-67 PMID: 3160483
  35. Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro.
    Cell. 1985 Oct;42(3):725-36 PMID: 2996774
  36. U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing.
    Cell. 1985 Oct;42(3):737-50 PMID: 2996775
  37. Localization of a base-paired interaction between small nuclear RNAs U4 and U6 in intact U4/U6 ribonucleoprotein particles by psoralen cross-linking.
    J Mol Biol. 1985 Oct 20;185(4):721-31 PMID: 2932555
  38. The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.
    Science. 1985 Dec 20;230(4732):1344-9 PMID: 2933810
  39. Polypeptide components of human small nuclear ribonucleoproteins.
    Proc Natl Acad Sci U S A. 1983 Sep;80(17):5208-12 PMID: 6225117
  40. Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein.
    Cell. 1983 Nov;35(1):101-7 PMID: 6194895
  41. Identification of human Sm and (U1) RNP antigens by immunoblotting.
    J Immunol Methods. 1983 Nov 11;64(1-2):45-59 PMID: 6227671
  42. Are U4 small nuclear ribonucleoproteins involved in polyadenylation?
    Nature. 1984 May 10-16;309(5964):179-82 PMID: 6325940
  43. U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle.
    Nucleic Acids Res. 1984 Apr 11;12(7):3283-93 PMID: 6201826
  44. Splicing pathways of SV40 mRNAs in X. laevis oocytes differ in their requirements for snRNPs.
    Cell. 1984 Jul;37(3):927-36 PMID: 6086149
  45. The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors.
    Cell. 1984 Aug;38(1):299-307 PMID: 6235919
  46. Intron splicing: a conserved internal signal in introns of animal pre-mRNAs.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7417-20 PMID: 6209716
  47. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.18.6721
Published
1986-09-00
Pages
6721-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386581
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.18.6721 PMC Full Text View journal details
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