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PMID: 29751673 Published · epublish English Journal Article

Expression of AhDREB1, an AP2/ERF Transcription Factor Gene from Peanut, Is Affected by Histone Acetylation and Increases Abscisic Acid Sensitivity and Tolerance to Osmotic Stress in Arabidopsis.

International journal of molecular sciences ·Vol. 19 ·No. 5 ·2018-05-11

Zhang B, Su L, Hu B, Li L

Abstract

Drought stress negatively affects plant growth and development. An increasing number of reports have revealed the involvement of APETALA2/Ethylene Responsive Factor (AP2/ERF) transcription factors (TFs) in biotic and abiotic stress regulation in plants. However, research on these TFs in the peanut plant (Arachis hypogaea) has been limited. Here, we isolated a full-length coding sequence (CDS) of the AP2/ERF family gene AhDREB1 from the peanut plant and showed that its expression was induced by Polyethylene Glycol (PEG) 6000 and exogenous abscisic acid (ABA) treatment. When overexpressed in Arabidopsis, AhDREB1 increased both ABA levels and ABA sensitivity, affected the ABA signaling pathway and increased the expression of downstream drought stress-related genes RD29A, P5CS1, P5CS2 and NCED1. These results demonstrate that AhDREB1 can improve tolerance to drought via the ABA-dependent pathway in Arabidopsis. In the peanut plant, the specific histone deacetylases (HDACs) inhibitor trichostatin A (TSA) promotes AhDREB1 transcription and the enrichment level of H3ac was increased in regions of the AhDREB1 gene during TSA and PEG treatment. In summary, histone acetylation can affect the expression of AhDREB1 under osmotic stress conditions, thereby improving plant drought resistance.

Keywords
APETALA2/Ethylene Responsive Factor AhDREB1 abscisic acid drought stresses histone acetylation peanut
MeSH Terms
Abscisic Acid/metabolism,pharmacology Acetylation Adaptation, Biological Amino Acid Sequence Arabidopsis/genetics,metabolism Arachis/genetics Cloning, Molecular Droughts Gene Expression Regulation, Plant Histones/metabolism Osmotic Pressure Signal Transduction/drug effects Stress, Physiological Transcription Factors/chemistry,genetics
Chemicals
Histones Transcription Factors Abscisic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zhang Baihong
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China. [email protected].
Su Liangchen
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China. [email protected]. | Department of Bioengineering, Zhuhai Campus of Zunyi Medical University, Zhuhai 519041, China. [email protected].
Hu Bo
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China. [email protected].
Li Ling
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China. [email protected].
Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Article Info
Journal
International journal of molecular sciences
Abbr.
Int J Mol Sci
ISSN
1422-0067
DOI
10.3390/ijms19051441
Email
[email protected]
[email protected]
[email protected]
[email protected]
Published
2018-05-11
Epub
2018-00-11
Language
English
Region
Switzerland
NLM ID
101092791
PMCID
PMC5983730
Subset
IM
External Links
PubMed source DOI: 10.3390/ijms19051441 PMC Full Text View journal details
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