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PMID: 2983312 Published · ppublish English Journal Article

Molecular cloning and complete sequence determination of RNA genome of human rhinovirus type 14.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 3 ·1985-02-00 ·Pages 732-6

Callahan PL, Mizutani S, Colonno RJ

Abstract

The genomic RNA of human rhinovirus type 14 was cloned in Escherichia coli and the complete nucleotide sequence was determined. The RNA genome is 7212 nucleotides long. A single large open reading frame of 6536 nucleotides was identified, which starts at nucleotide 678 and ends 47 nucleotides from the 3' end of the RNA genome. Comparisons of the specified proteins with those of other picornaviruses showed a striking homology (44-65%) between rhinovirus and poliovirus. The rhinovirus genomic RNA is rich in adenosine (32.1%) and strongly favors an adenosine or uridine in the third position of codons. The predicted map locations of all the rhinovirus structural and non-structural proteins and their proposed proteolytic cleavage sites are described.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Deletion Cloning, Molecular DNA/analysis Protein Biosynthesis RNA, Viral/analysis Rhinovirus/genetics Software
Chemicals
RNA, Viral DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Callahan P L
Mizutani S
Colonno R J
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.3.732
Published
1985-02-00
Pages
732-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397120
Subset
IM
Databases
GENBANK
K02121, X01087
External Links
PubMed source DOI: 10.1073/pnas.82.3.732 PMC Full Text View journal details
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