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PMID: 2987828 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Topoisomerase I has a strong binding preference for a conserved hexadecameric sequence in the promoter region of the rRNA gene from Tetrahymena pyriformis.

Nucleic acids research ·Vol. 13 ·No. 5 ·1985-03-11 ·Pages 1543-57

Andersen AH, Gocke E, Bonven BJ, Nielsen OF, Westergaard O

Abstract

Topoisomerase I is in situ associated with DNaseI hypersensitive sites located in the promotor and terminator regions of the extrachromosomal rDNA in Tetrahymena thermophila at sites with sequences fitting the motif (sequence in text) Reconstitution experiments with purified topoisomerase I and cloned fragments of rDNA demonstrate that the enzyme exhibits the same binding and cleavage properties on naked DNA. These observations are striking as topoisomerase I previously has been found to exhibit low sequence specificity. The specific binding of the enzyme has an absolute requirement for divalent cations with a preference for Ca2+. The strong binding to the hexadecamer has been characterized by competition experiments, and it has been used to determine the molecular weight of the enzyme.

MeSH Terms

Animals Base Sequence Calcium/metabolism DNA Restriction Enzymes/metabolism DNA Topoisomerases, Type I/metabolism Deoxyribonuclease I/metabolism Isoenzymes/metabolism Magnesium/metabolism Molecular Weight Operon RNA, Ribosomal/genetics Structure-Activity Relationship Substrate Specificity Tetrahymena pyriformis/enzymology,genetics

Chemicals

Isoenzymes RNA, Ribosomal DNA Restriction Enzymes Deoxyribonuclease I DNA Topoisomerases, Type I Magnesium Calcium

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Andersen A H

Gocke E

Bonven B J

Nielsen O F

Westergaard O

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23 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/13.5.1543

Published

1985-03-11

Pages

1543-57

Language

English

Region

England

NLM ID

0411011

PMCID

PMC341095

Subset

External Links

PubMed source DOI: 10.1093/nar/13.5.1543 PMC Full Text View journal details

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Topoisomerase I has a strong binding preference for a conserved hexadecameric sequence in the promoter region of the rRNA gene from Tetrahymena pyriformis.

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