Home LiteratureArticle Details
PMID: 2997750 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The genomic organisation and nucleotide sequence of the HLA-SB(DP) alpha gene.

Nucleic acids research ·Vol. 13 ·No. 20 ·1985-10-25 ·Pages 7515-28

Lawrance SK, Das HK, Pan J, Weissman SM

Abstract

We have isolated a unique fragment of the HLA-DR alpha gene and probed human genomic DNA at low stringency to search for homologous sequences. A minimum of six non-polymorphic cross-hybridizing high molecular weight fragments were found in all DNAs examined. In order to obtain molecular clones of these cross-hybridizing fragments, we constructed lambda and cosmid libraries of human DNA and screened them at low stringency with the HLA-DR alpha gene specific subclone. We have isolated clones corresponding to each of the six fragments and, in this paper, describe those which contain the gene encoding HLA-SB(DP) alpha.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Evolution Cloning, Molecular DNA Restriction Enzymes Genes HLA-DP Antigens HLA-DR Antigens Histocompatibility Antigens Class II/genetics Humans Immunoglobulin epsilon-Chains/genetics Sequence Homology, Nucleic Acid
Chemicals
HLA-DP Antigens HLA-DR Antigens Histocompatibility Antigens Class II Immunoglobulin epsilon-Chains DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lawrance S K
Das H K
Pan J
Weissman S M
References (31)
31 references, click to expand
  1. DNA sequence and characterization of human class II major histocompatibility complex beta chains from the DR1 haplotype.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3405-9 PMID: 3858829
  2. Complete nucleotide sequence of a functional HLA-DP beta gene and the region between the DP beta 1 and DP alpha 1 genes: comparison of the 5' ends of HLA class II genes.
    Nucleic Acids Res. 1985 Mar 11;13(5):1607-21 PMID: 2987832
  3. SB subregion of the human major histocompatibility complex: gene organization, allelic polymorphism and expression in transformed cells.
    EMBO J. 1985 Mar;4(3):739-48 PMID: 2988934
  4. A general method for isolation of high molecular weight DNA from eukaryotes.
    Nucleic Acids Res. 1976 Sep;3(9):2303-8 PMID: 987581
  5. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.
    Science. 1977 Apr 8;196(4286):175-7 PMID: 322278
  6. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  7. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  8. In vitro packaging of lambda and cosmid DNA.
    Methods Enzymol. 1979;68:299-309 PMID: 161604
  9. Evidence for a new segregant series of B cell antigens that are encoded in the HLA-D region and that stimulate secondary allogenic proliferative and cytotoxic responses.
    J Exp Med. 1980 Sep 1;152(3):565-80 PMID: 6967946
  10. Construction of coliphage lambda Charon vectors with BamHI cloning sites.
    Gene. 1980 Dec;12(3-4):301-9 PMID: 6265323
  11. HLA-linked B cell alloantigens of a new segregant series: population and family studies of the SB antigens.
    Hum Immunol. 1980 Sep;1(2):177-85 PMID: 6973559
  12. Rapid and efficient cosmid cloning.
    Nucleic Acids Res. 1981 Jul 10;9(13):2989-98 PMID: 6269067
  13. Gene dosage and gene expression in the HLA region: evidence from deletion variants.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1235-9 PMID: 6951171
  14. Alpha and beta chains of SB and DR antigens are structurally distinct.
    J Exp Med. 1982 Nov 1;156(5):1557-62 PMID: 6182264
  15. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.
    Nucleic Acids Res. 1982 Aug 11;10(15):4731-51 PMID: 7133997
  16. SB-restricted presentation of influenza and herpes simplex virus antigens to human T-lymphocyte clones.
    Nature. 1983 Feb 24;301(5902):716-8 PMID: 6186920
  17. Use of synthetic oligonucleotide probes complementary to genes for human HLA-DR alpha and beta as extension primers for the isolation of 5'-specific genomic clones.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1531-5 PMID: 6403940
  18. Structure of the human immunoglobulin C epsilon 2 gene, a truncated pseudogene: implications for its evolutionary origin.
    Proc Natl Acad Sci U S A. 1983 May;80(10):2995-9 PMID: 6407005
  19. Structure and nucleotide sequence of the heavy chain gene of HLA-DR.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3543-7 PMID: 6304715
  20. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  21. Mapping SB in relation to HLA and GLO1 using cells from first-cousin marriage offspring.
    Immunogenetics. 1983;18(5):503-12 PMID: 6417008
  22. The class II molecules of the human and murine major histocompatibility complex.
    Cell. 1984 Jan;36(1):1-13 PMID: 6198089
  23. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  24. Isotypic and allotypic variation of human class II histocompatibility antigen alpha-chain genes.
    Nature. 1984 Mar 22-28;308(5957):327-33 PMID: 6584734
  25. Molecular organization of the HLA-SB region of the human major histocompatibility complex and evidence for two SB beta-chain genes.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):3934-8 PMID: 6330724
  26. Selective isolation of cosmid clones by homologous recombination in Escherichia coli.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):4129-33 PMID: 6330743
  27. Linkage map of two HLA-SB beta and two HLA-SB alpha-related genes: an intron in one of the SB beta genes contains a processed pseudogene.
    Cell. 1984 Aug;38(1):241-9 PMID: 6088068
  28. Structure and polymorphism of the HLA class II SB light chain genes.
    EMBO J. 1984 Dec 1;3(12):2985-93 PMID: 6098459
  29. Molecular map of the human HLA-SB (HLA-DP) region and sequence of an SB alpha (DP alpha) pseudogene.
    EMBO J. 1984 Dec 20;3(13):3209-14 PMID: 6597088
  30. The molecular genetics of human hemoglobin.
    Prog Nucleic Acid Res Mol Biol. 1984;31:315-462 PMID: 6397774
  31. Gene organization of DC and DX subregions of the human major histocompatibility complex.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3410-4 PMID: 3858830
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.20.7515
Published
1985-10-25
Pages
7515-28
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322059
Subset
IM
Grants
NIADDK NIH HHS · AM 33871-02 · United States
Databases
GENBANK
X03100
External Links
PubMed source DOI: 10.1093/nar/13.20.7515 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2