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PMID: 3012569 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High-resolution analysis of the human HLA-DR polymorphism by hybridization with sequence-specific oligonucleotide probes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 12 ·1986-06-00 ·Pages 4489-93

Angelini G, de Preval C, Gorski J, Mach B

Abstract

The human major histocompatibility complex class II antigens of the HLA-D are highly polymorphic, surface proteins essential in the cellular interactions necessary for an immune response. The analysis of this polymorphism is crucial for (i) histocompatibility matching for transplantation and (ii) understanding the association between HLA-D and certain important diseases. The polymorphism of certain HLA-D haplotypes may escape detection by current methodologies. Analysis at the genomic level of the polymorphism of one of the HLA-D subregions HLA-DR, using oligonucleotide probes specific for the polymorphic regions, is capable of distinguishing single nucleotide differences. The DRw6 haplotype was analyzed in view of the lack of DRw6 specific sera. On the basis of nucleotide sequence analysis, the DRw6 haplotype consists of at least two subtypes. When analyzed with oligonucleotide probes, this split identifies new polymorphic groups that differ from the DRw6 serological subgroups.

MeSH Terms
DNA Restriction Enzymes Genes HLA-DR Antigens Histocompatibility Antigens Class II/genetics Humans Nucleic Acid Hybridization Oligodeoxyribonucleotides/chemical synthesis Polymorphism, Genetic
Chemicals
HLA-DR Antigens Histocompatibility Antigens Class II Oligodeoxyribonucleotides DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Angelini G
de Preval C
Gorski J
Mach B
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18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.12.4489
Published
1986-06-00
Pages
4489-93
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323759
Subset
IM
Databases
GENBANK
M13561, M13562
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1073/pnas.83.12.4489 PMC Full Text View journal details
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