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PMID: 3023895 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transformation of chicken embryo fibroblasts and tumor induction by the middle T antigen of polyomavirus carried in an avian retroviral vector.

Molecular and cellular biology ·Vol. 6 ·No. 5 ·1986-05-00 ·Pages 1545-51

Kornbluth S, Cross FR, Harbison M, Hanafusa H

Abstract

The middle T antigen of polyomavirus transformed primary chicken embryo fibroblasts when expressed from a replication-competent avian retrovirus. This in vitro-constructed retrovirus, SRMT1, is a variant of Rous sarcoma virus that encodes the middle T antigen in place of v-src. Inoculation of SRMT1 into 1-week-old chickens rapidly induced hemangiomas and hemangiosarcomas. As shown with mammalian cells infected with polyomavirus, polyomavirus middle T antigen appears to be associated with p60c-src in chicken cells infected with SRMT1. When lysates of SRMT1-infected cells immunoprecipitated with either a monoclonal antibody against p60src or anti-T serum were assayed in an in vitro kinase reaction, the middle T antigen was heavily phosphorylated. To see whether an excess of p60c-src could alter the extent of phosphorylation of the middle T protein or the process of cell transformation by middle T, cells were doubly infected with SRMT1 and NY501, a virus which overexpresses p60c-src. Doubly infected chicken embryo fibroblasts transformed with the same kinetics and were morphologically indistinguishable from chicken embryo fibroblasts infected with SRMT1 alone. Phosphorylation of the middle T antigen was elevated two- to fivefold relative to cells infected only with SRMT1.

MeSH Terms
Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/genetics Avian Sarcoma Viruses/genetics Cell Transformation, Neoplastic Chick Embryo Chickens Fibroblasts Genetic Vectors Oncogene Proteins, Viral/genetics Plasmids Polyomavirus/genetics Protein Kinases/genetics Rats Sarcoma, Avian/pathology Transfection
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Oncogene Proteins, Viral Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kornbluth S
Cross F R
Harbison M
Hanafusa H
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43 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.5.1545-1551.1986
Published
1986-05-00
Pages
1545-51
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367680
Subset
IM
Grants
NCI NIH HHS · CA14935 · United States
NCI NIH HHS · CA18213 · United States
NCRR NIH HHS · RR01180 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.5.1545-1551.1986 PMC Full Text View journal details
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