Home LiteratureArticle Details

PMID: 3025084 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Extensive deletions in the Q region of the mouse major histocompatibility complex.

Immunogenetics ·Vol. 24 ·No. 6 ·1986-00-00 ·Pages 368-73

O'Neill AE, Reid K, Garberi JC, Karl M, Flaherty L

Abstract

By use of Southern blot analyses and low copy number probes, the fine structure of the Q region of the mouse major histocompatibility complex was studied in more detail. With a probe recognizing the even-numbered genes Q4, Q6, and Q8, it was evident that Q4 and/or the regions flanking Q4 are polymorphic, whereas Q6 and Q8, and their flanking regions are nonpolymorphic. Perhaps the most noteworthy finding is that at least two strain haplotypes, H-2k and H-2f, possessed extensive deletions in the Q region. The most striking deletion was found in the H-2f haplotype, where the Q1 through Q9 genes appear to be missing. Because of these extensive deletions the functional importance of the Q region is questioned.

MeSH Terms

Animals Chromosome Deletion Cosmids DNA/isolation & purification DNA Restriction Enzymes Major Histocompatibility Complex Mice Mice, Inbred Strains Nucleic Acid Hybridization

Chemicals

DNA DNA Restriction Enzymes

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

O'Neill A E

Reid K

Garberi J C

Karl M

Flaherty L

References (29)

29 references, click to expand

Unusual association of beta 2-microglobulin with certain class I heavy chains of the murine major histocompatibility complex.
Proc Natl Acad Sci U S A. 1986 Jan;83(2):432-6 PMID: 3510435
Occurrence of a unique MHC class I gene in distantly related members of the genus Mus.
Immunogenetics. 1985;21(3):267-76 PMID: 3988322
Qb-1, a new class I polypeptide encoded by the Qa region of the mouse H-2 complex.
Immunogenetics. 1985;22(3):285-9 PMID: 4044021
Structure and expression of genes encoding murine Qa-2 class I antigens.
Proc Natl Acad Sci U S A. 1985 Sep;82(17):5920-4 PMID: 3862106
Genetic mapping in the major histocompatibility complex by restriction enzyme site polymorphisms: most mouse class I genes map to the Tla complex.
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3425-9 PMID: 6304710
Gene duplications in the TL region of the mouse major histocompatibility complex.
EMBO J. 1985 Jun;4(6):1431-4 PMID: 4029119
Genetics of the major histocompatibility complex: the final act.
Annu Rev Immunol. 1983;1:119-42 PMID: 6399975
Tissue-specific expression of an unusual H-2 (class I)-related gene.
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4947-51 PMID: 6956903
A nonpolymorphic class I gene in the murine major histocompatibility complex.
Cell. 1984 Jan;36(1):139-44 PMID: 6420070
Expression of class I genes in the major histocompatibility complex: identification of eight distinct mRNAs in DBA/2 mouse liver.
Cell. 1985 Jun;41(2):469-78 PMID: 3838699
Characteristics of the expression of the murine soluble class I molecule (Q10).
J Immunol. 1986 Jan;136(1):254-8 PMID: 3940270
Family organization of mouse H-2 class I genes.
Immunogenetics. 1985;21(4):343-53 PMID: 3997208
Polymorphism of Qa and Tla loci of the mouse.
Tissue Antigens. 1983 Sep;22(3):204-12 PMID: 6636113
Qa gene expression: biosynthesis and secretion of Qa-2 molecules in activated T cells.
Proc Natl Acad Sci U S A. 1986 May;83(9):2949-53 PMID: 3486423
The class I MHC gene family in mice.
Immunol Today. 1986 Jan;7(1):19-24 PMID: 25291420
Clusters of genes encoding mouse transplantation antigens.
Cell. 1982 Mar;28(3):489-98 PMID: 6280871
Further polymorphism of the Tla locus defined by monoclonal TL antibodies.
Immunogenetics. 1982;15(6):573-8 PMID: 7106865
Polytenization of the ribosomal genes on the X and Y chromosomes of Drosophila melanogaster.
Genetics. 1982 Mar;100(3):375-85 PMID: 6811371
Secretion of a transplantation-related antigen.
Cell. 1983 Aug;34(1):189-96 PMID: 6411351
Secretion of a soluble class I molecule encoded by the Q10 gene of the C57BL/10 mouse.
EMBO J. 1985 Feb;4(2):369-74 PMID: 4018029
Genes of the major histocompatibility complex of the mouse.
Annu Rev Immunol. 1983;1:529-68 PMID: 6152713
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
Crossreactivity between Qa-1 region and H-2K antigens.
Transplantation. 1983 Apr;35(4):391-3 PMID: 6836711
Characterization of a Q subregion gene in the murine major histocompatibility complex.
Proc Natl Acad Sci U S A. 1985 Mar;82(5):1503-7 PMID: 2983348
A general method for isolation of high molecular weight DNA from eukaryotes.
Nucleic Acids Res. 1976 Sep;3(9):2303-8 PMID: 987581
Serologic cross-reactivity between Class I MHC molecules and an H-2-linked differentiation antigen as detected by monoclonal antibodies.
J Exp Med. 1984 Jan 1;159(1):21-40 PMID: 6363595
Qa-2 does not display structural genetic polymorphism detectable on isoelectric-focusing gels.
Immunogenetics. 1982;16(4):363-6 PMID: 7174001
Organization and evolution of the class I gene family in the major histocompatibility complex of the C57BL/10 mouse.
Nature. 1984 Aug 23-29;310(5979):650-5 PMID: 6088985
The Qa-1 alloantigens. II. Evidence for the expression of two Qa-1 molecules by the Qa-1d genotype and for cross-reactivity between Qa-1 and H-2K.
J Immunol. 1983 Mar;130(3):1293-9 PMID: 6822734

Article Info

Journal

Immunogenetics

Abbr.

Immunogenetics

ISSN

0093-7711

DOI

10.1007/BF00377954

Published

1986-00-00

Pages

368-73

Language

English

Region

United States

NLM ID

0420404

Subset

Grants

NCI NIH HHS · CA35055 · United States

NIGMS NIH HHS · GM37947 · United States

External Links

PubMed source DOI: 10.1007/BF00377954 View journal details

Analysis Services

Extensive deletions in the Q region of the mouse major histocompatibility complex.

Contact

WeChat Official Account