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PMID: 3025182 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcription control of the aroP gene in Escherichia coli K-12: analysis of operator mutants.

Journal of bacteriology ·Vol. 169 ·No. 1 ·1987-01-00 ·Pages 386-93

Chye ML, Pittard J

Abstract

The nucleotide sequence of the region containing the promoter-operator for the aroP gene was determined. The start site of aroP transcription was identified by using S1 nuclease mapping and primer extension techniques. Examination of the nucleotide sequence revealed the presence of two "TYR R" boxes which are similar to those identified in the regulatory regions of other genes in the tyrR regulon. Bisulfite-induced aroP operator-constitutive mutants were analyzed, and the base-pair changes responsible for alterations in aroP regulation were located within these boxes.

MeSH Terms
Acetyltransferases/metabolism Base Sequence Chloramphenicol O-Acetyltransferase Electrophoresis, Polyacrylamide Gel Endonucleases/metabolism Escherichia coli/genetics Genes, Bacterial Mutation Operon Promoter Regions, Genetic Single-Strand Specific DNA and RNA Endonucleases Sulfites/pharmacology Transcription, Genetic
Chemicals
Sulfites Acetyltransferases Chloramphenicol O-Acetyltransferase Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chye M L
Pittard J
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.1.386-393.1987
Published
1987-01-00
Pages
386-93
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211779
Subset
IM
Databases
GENBANK
M15312
External Links
PubMed source DOI: 10.1128/jb.169.1.386-393.1987 PMC Full Text View journal details
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