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PMID: 3025598 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Overproduction of protein p53 contributes to simian virus 40-mediated transformation.

Molecular and cellular biology ·Vol. 6 ·No. 10 ·1986-10-00 ·Pages 3531-6

Michalovitz D, Eliyahu D, Oren M

Abstract

The possible involvement of p53 overproduction in simian virus 40 (SV40)mediated transformation was studied by using the rat embryo fibroblast focus formation assay. Transformation by wild-type SV40 was enhanced two- to threefold by cotransfection of a plasmid overexpressing mouse p53. More significantly, such a plasmid could partially complement a transformation-defective deletion mutant of SV40. Hence, the ability of SV40 T antigen to induce high p53 levels may indeed be directly relevant to the viral transforming potential.

MeSH Terms
Animals Cell Transformation, Viral Cells, Cultured Genes Genes, Viral Mice Mice, Inbred Strains Mutation Neoplasm Proteins/genetics Phosphoproteins/genetics Simian virus 40/genetics Transfection Tumor Suppressor Protein p53
Chemicals
Neoplasm Proteins Phosphoproteins Tumor Suppressor Protein p53
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Michalovitz D
Eliyahu D
Oren M
References (37)
37 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.10.3531-3536.1986
Published
1986-10-00
Pages
3531-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367102
Subset
IM
Grants
NCI NIH HHS · R01 CA 40099-01 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.10.3531-3536.1986 PMC Full Text View journal details
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