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PMID: 3029685 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus.

Nucleic acids research ·Vol. 15 ·No. 2 ·1987-01-26 ·Pages 595-609

Beck TW, Huleihel M, Gunnell M, Bonner TI, Rapp UR

Abstract

The complete 606 amino acid sequence of the human A-raf oncogene has been deduced from the 2453 nucleotide sequence of a human T cell cDNA. A cysteine-rich region located near the amino terminus, which is highly conserved in A-raf and c-raf, shows significant homology with protein kinase C. A 5' deleted fragment of the cDNA has been incorporated into a murine retrovirus which endows the virus with the ability to transform cells in vivo and in vitro. Functionally, human A-raf is similar to v-raf and v-mos in that transformation is independent of ras gene function.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Transformation, Neoplastic DNA/analysis Genes, Viral Humans Liver/embryology Mice Moloney murine sarcoma virus/genetics Oncogenes Sarcoma Viruses, Murine/genetics T-Lymphocytes/analysis
Chemicals
DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Beck T W
Huleihel M
Gunnell M
Bonner T I
Rapp U R
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42 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.2.595
Published
1987-01-26
Pages
595-609
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340454
Subset
IM
Grants
NCI NIH HHS · N01-CO-23910 · United States
Databases
GENBANK
X04790
External Links
PubMed source DOI: 10.1093/nar/15.2.595 PMC Full Text View journal details
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