Home LiteratureArticle Details
PMID: 3031604 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Aggregation of DNA by analogs of spermidine; enzymatic and structural studies.

Nucleic acids research ·Vol. 15 ·No. 6 ·1987-03-25 ·Pages 2563-80

Srivenugopal KS, Wemmer DE, Morris DR

Abstract

A homologous series of spermidine analogs, with defined abilities to replace the natural polyamine in supporting cell growth, was examined for its influence on the structure of supercoiled, aggregated DNA and on the ability of the DNA aggregates to act as substrates for various enzymes. The concentration of amine necessary to aggregate negatively supercoiled Col E1 DNA was progressively increased as the diaminobutane moiety of spermidine was extended beyond 5 methylene groups. 1H- and 31P-NMR spectroscopy suggested that less rigid DNA aggregates were formed by spermidine analogs than by spermidine itself. Spermidine and its analogs differentially modulated the activities of bacterial and mammalian type I topoisomerases and EcoRI restriction endonuclease on aggregated DNA in a manner reminiscent of the abilities of the amines to stimulate cell growth. When DNA was not aggregated, the influence of the various amines on these reactions was almost identical. These results are discussed in relation to the structures of the DNA aggregates in the presence of the various triamines.

MeSH Terms
Animals Cattle DNA DNA Topoisomerases, Type I DNA Topoisomerases, Type II Endonucleases Escherichia coli/enzymology Kinetics Macromolecular Substances Magnetic Resonance Spectroscopy Single-Strand Specific DNA and RNA Endonucleases Spermidine/analogs & derivatives Structure-Activity Relationship Thymus Gland/enzymology
Chemicals
Macromolecular Substances DNA Endonucleases Single-Strand Specific DNA and RNA Endonucleases DNA Topoisomerases, Type I DNA Topoisomerases, Type II Spermidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Srivenugopal K S
Wemmer D E
Morris D R
References (46)
46 references, click to expand
  1. Formation of a compensatory polyamine by Escherichia coli polyamine-requiring mutants during growth in the absence of polyamines.
    J Bacteriol. 1986 Apr;166(1):128-34 PMID: 3514574
  2. Functional compartmentation of intracellular magnesium.
    Magnesium. 1986;5(2):53-9 PMID: 3086631
  3. Modulation of the relaxing activity of Escherichia coli topoisomerase I by single-stranded DNA binding proteins.
    Biochem Biophys Res Commun. 1986 Jun 13;137(2):795-800 PMID: 3015129
  4. On the toroidal condensed state of closed circular DNA.
    J Biomol Struct Dyn. 1985 Dec;3(3):515-20 PMID: 3917035
  5. An X-ray study of the interaction of DNA with spermine.
    J Mol Biol. 1969 Jun 14;42(2):363-73 PMID: 5803301
  6. 1,4-Diaminobutane (putrescine), spermidine, and spermine.
    Annu Rev Biochem. 1976;45:285-306 PMID: 786151
  7. DNA gyrase: an enzyme that introduces superhelical turns into DNA.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3872-6 PMID: 186775
  8. Spermidine-Deoxyribonucleic acid interaction in vitro and in Escherichia coli.
    J Bacteriol. 1977 Feb;129(2):916-25 PMID: 320196
  9. Polyamine induced aggregation of DNA.
    Nucleic Acids Res. 1977 Mar;4(3):685-95 PMID: 17099
  10. The compaction of DNA helices into either continuous supercoils or folded-fiber rods and toroids.
    Cell. 1978 Feb;13(2):295-306 PMID: 203402
  11. Massive airway hemorrhage after transtracheal aspiration.
    South Med J. 1978 Mar;71(3):325-7 PMID: 628856
  12. DNA condensation with polyamines I. Spectroscopic studies.
    J Mol Biol. 1978 May 25;121(3):311-26 PMID: 671540
  13. The molecular theory of polyelectrolyte solutions with applications to the electrostatic properties of polynucleotides.
    Q Rev Biophys. 1978 May;11(2):179-246 PMID: 353876
  14. Counterion-induced condesation of deoxyribonucleic acid. a light-scattering study.
    Biochemistry. 1979 May 29;18(11):2192-6 PMID: 444448
  15. Mutants of Escherichia coli that do not contain 1,4-diaminobutane (putrescine) or spermidine.
    J Biol Chem. 1979 Dec 25;254(24):12419-26 PMID: 159306
  16. Relationship between S1 endonuclease-sensitivity and number of superhelical turns in a negatively-twisted DNA.
    FEBS Lett. 1980 Mar 10;111(2):333-6 PMID: 6244188
  17. Structural specificity of the spermidine requirement of an Escherichia coli auxotroph.
    J Bacteriol. 1980 Feb;141(2):456-63 PMID: 6154032
  18. Stimulation of deoxyribonucleic acid replication fork movement by spermidine analogs in polyamine-deficient Escherichia coli.
    J Bacteriol. 1980 Mar;141(3):1192-8 PMID: 6988409
  19. Internal motions in deoxyribonucleic acid II.
    Biochemistry. 1980 Jul 22;19(15):3460-8 PMID: 7190834
  20. Specificity of the spermidine requirement for the replication of phi X174 DNA be cell-free extracts of Escherichia coli.
    Biochim Biophys Acta. 1980 Sep 19;609(2):264-71 PMID: 6447515
  21. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  22. The inverted repeat as a recognizable structural feature in supercoiled DNA molecules.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6468-72 PMID: 6256738
  23. Structure of viral phi 29 DNA condensed by simple triamines: a light-scattering and electron-microscopy study.
    Biopolymers. 1981 Mar;20(3):469-88 PMID: 7213939
  24. The interaction of polyamines with DNA: a 23Na NMR study.
    Nucleic Acids Res. 1981 Mar 11;9(5):1219-28 PMID: 7232215
  25. Cation-induced toroidal condensation of DNA studies with Co3+(NH3)6.
    J Mol Biol. 1980 Dec 25;144(4):431-53 PMID: 6454789
  26. Purification and properties of the Escherichia coli protein factor required for lambda integrative recombination.
    J Biol Chem. 1981 Sep 10;256(17):9246-53 PMID: 6267068
  27. Studies on the selectivity of DNA precipitation by spermine.
    Nucleic Acids Res. 1981 Oct 24;9(20):5493-504 PMID: 7029471
  28. The effect of several nucleic acid binding drugs on the cleavage of d(GGAATTCC) and pBR 322 by the Eco RI restriction endonuclease.
    Nucleic Acids Res. 1981 Nov 25;9(22):6115-27 PMID: 6273824
  29. Catenation of DNA rings by topoisomerases. Mechanism of control by spermidine.
    J Biol Chem. 1982 Mar 10;257(5):2687-93 PMID: 6277910
  30. Relationship between superhelical density and cruciform formation in plasmid pVH51.
    J Biol Chem. 1982 Jun 10;257(11):6292-5 PMID: 6281266
  31. Spermidine requirement for cell proliferation in eukaryotic cells: structural specificity and quantitation.
    Science. 1983 Mar 4;219(4588):1083-5 PMID: 6823570
  32. Ion competition and micrococcal nuclease digestion studies of spermidine-condensed calf thymus DNA. Evidence for torus organization by circumferential DNA wrapping.
    Biochim Biophys Acta. 1983 Dec 22;741(3):279-87 PMID: 6652092
  33. DNA topoisomerases--enzymes that catalyse the breaking and rejoining of DNA.
    CRC Crit Rev Biochem. 1983;15(1):1-24 PMID: 6319080
  34. DNA: sequence, structure and supercoiling. The twentieth Colworth medal lecture.
    Biochem Soc Trans. 1984 Apr;12(2):127-40 PMID: 6327420
  35. X-ray diffraction studies on cation-collapsed DNA.
    J Mol Biol. 1984 May 25;175(3):313-29 PMID: 6726812
  36. Internal motion of DNA in bacteriophages.
    J Biol Chem. 1984 Jul 10;259(13):8338-44 PMID: 6234311
  37. Ionic and structural effects on the thermal helix-coil transition of DNA complexed with natural and synthetic polyamines.
    Biopolymers. 1984 Jul;23(7):1295-306 PMID: 6466769
  38. Polyamines.
    Annu Rev Biochem. 1984;53:749-90 PMID: 6206782
  39. Dynamics of DNA condensation.
    Biochemistry. 1984 Oct 9;23(21):4821-8 PMID: 6238622
  40. Effect of polyamines and basic proteins on cleavage of DNA by restriction endonucleases.
    Biochemistry. 1984 Nov 20;23(24):5697-703 PMID: 6098296
  41. Spermidine increases the accuracy of type II restriction endonucleases. Suppression of cleavage at degenerate, non-symmetrical sites.
    Eur J Biochem. 1985 Feb 15;147(1):105-9 PMID: 2982605
  42. Phosphorus-31 nuclear magnetic resonance of highly oriented DNA fibers. 2. Molecular motions in hydrated DNA.
    Biochemistry. 1985 Feb 12;24(4):896-902 PMID: 3994998
  43. Differential effects on the B-to-Z transition of poly(dG-me5dC).poly(dG-me5dC) produced by N1- and N8-acetyl spermidine.
    Biopolymers. 1985 Apr;24(4):725-9 PMID: 3995158
  44. Nucleotide sequence and gene organization of ColE1 DNA.
    J Biol Chem. 1985 Jul 25;260(15):8925-35 PMID: 2991225
  45. Nuclear magnetic resonance studies of polyamine binding to a defined DNA sequence.
    J Mol Biol. 1985 Sep 20;185(2):457-9 PMID: 4057253
  46. Differential modulation by spermidine of reactions catalyzed by type 1 prokaryotic and eukaryotic topoisomerases.
    Biochemistry. 1985 Aug 27;24(18):4766-71 PMID: 3000418
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.6.2563
Published
1987-03-25
Pages
2563-80
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340669
Subset
IM
Grants
NIGMS NIH HHS · GM28764-01S1 · United States
External Links
PubMed source DOI: 10.1093/nar/15.6.2563 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2