Home LiteratureArticle Details
PMID: 3038515 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Localization of the mcf.2 transforming sequence to the X chromosome.

The EMBO journal ·Vol. 6 ·No. 5 ·1987-05-00 ·Pages 1301-7

Noguchi T, Mattei MG, Oberlè I, Planche J, Imbert J, Pelassy C, Birg F, Birnbaum D

Abstract

A transforming sequence was identified using co-transfection of DNA from the human mammary carcinoma cell line MCF-7 and of a G418 resistance gene into NIH 3T3 cells, followed by tumor formation in athymic mice. This sequence, named mcf.2, was molecularly cloned. A transforming activity resides in a cosmid clone of 42 kb. mcf.2 did not cross-hybridize with the known oncogenes tested. In situ hybridization localized it on the X chromosome, probably at q27. This localization was confirmed by hybridization to a panel of human--rodent cell line DNAs.

MeSH Terms
Animals Breast Neoplasms Cell Line Cell Transformation, Neoplastic Chromosome Mapping Cloning, Molecular Cosmids DNA Restriction Enzymes Female Humans Hybrid Cells/cytology Nucleic Acid Hybridization RNA, Neoplasm/genetics X Chromosome
Chemicals
RNA, Neoplasm DNA Restriction Enzymes
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Noguchi T
Mattei M G
Oberlè I
Planche J
Imbert J
Pelassy C
Birg F
Birnbaum D
References (33)
33 references, click to expand
  1. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  2. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  3. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
    Anal Biochem. 1976 Jan;70(1):75-85 PMID: 1259158
  4. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase.
    Int J Cancer. 1977 Jan;19(1):128-35 PMID: 832918
  5. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity.
    Science. 1978 Jun 9;200(4346):1153-4 PMID: 306682
  6. X-linked mental retardation with macro-orchidism and the fragile site at Xq 27 or 28.
    Hum Genet. 1979 Apr 17;48(1):117-20 PMID: 572332
  7. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  8. A small cosmid for efficient cloning of large DNA fragments.
    Gene. 1980 Nov;11(3-4):291-8 PMID: 6260575
  9. Identification of a phosphoprotein specifically induced by the transforming DNA of rat neuroblastomas.
    Cell. 1982 Apr;28(4):865-71 PMID: 7094016
  10. Mechanism of activation of a human oncogene.
    Nature. 1982 Nov 11;300(5888):143-9 PMID: 6290897
  11. A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene.
    Nature. 1982 Nov 11;300(5888):149-52 PMID: 7133135
  12. Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change.
    Nature. 1982 Dec 23;300(5894):762-5 PMID: 7177195
  13. Three human transforming genes are related to the viral ras oncogenes.
    Proc Natl Acad Sci U S A. 1983 Apr;80(8):2112-6 PMID: 6572964
  14. Dispersion of the ras family of transforming genes to four different chromosomes in man.
    Nature. 1983 Apr 28;302(5911):839-42 PMID: 6843651
  15. Structure of the Ki-ras gene of the human lung carcinoma cell line Calu-1.
    Nature. 1983 Aug 11-17;304(5926):497-500 PMID: 6308465
  16. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  17. The human c-Ha-ras2 is a processed pseudogene inactivated by numerous base substitutions.
    Nucleic Acids Res. 1984 Feb 24;12(4):1821-8 PMID: 6322122
  18. New human transforming genes detected by a tumorigenicity assay.
    Mol Cell Biol. 1984 Sep;4(9):1695-705 PMID: 6092933
  19. Expression of the c-fos gene and of an fos-related gene is stimulated by platelet-derived growth factor.
    Science. 1984 Nov 30;226(4678):1080-2 PMID: 6093261
  20. DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome.
    Hum Genet. 1985;69(3):268-71 PMID: 3980018
  21. Isolation of a new human oncogene from a diffuse B-cell lymphoma.
    Nature. 1985 Jul 18-24;316(6025):273-5 PMID: 3875039
  22. Activation of a novel human transforming gene, ret, by DNA rearrangement.
    Cell. 1985 Sep;42(2):581-8 PMID: 2992805
  23. Characterization of a set of X-linked sequences and of a panel of somatic cell hybrids useful for the regional mapping of the human X chromosome.
    Hum Genet. 1986 Jan;72(1):43-9 PMID: 3002952
  24. A human oncogene formed by the fusion of truncated tropomyosin and protein tyrosine kinase sequences.
    Nature. 1986 Feb 27-Mar 5;319(6056):743-8 PMID: 2869410
  25. Genetic analysis of the fragile-X mental retardation syndrome with two flanking polymorphic DNA markers.
    Proc Natl Acad Sci U S A. 1986 Feb;83(4):1016-20 PMID: 3006023
  26. Isolation and characterization of a new cellular oncogene encoding a protein with multiple potential transmembrane domains.
    Cell. 1986 Jun 6;45(5):711-9 PMID: 3708691
  27. Actively transcribed genes in the raf oncogene group, located on the X chromosome in mouse and human.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3934-8 PMID: 3520560
  28. Characterization of an activated human ros gene.
    Mol Cell Biol. 1986 Sep;6(9):3109-16 PMID: 3785223
  29. Human-tumor-derived cell lines contain common and different transforming genes.
    Cell. 1981 Dec;27(3 Pt 2):467-76 PMID: 6101201
  30. Co-transfection of normal NIH/3T3 DNA and retroval LTR sequences: a novel strategy for the detection of potential c-onc genes.
    EMBO J. 1984 May;3(5):1121-7 PMID: 6329737
  31. Molecular cloning of a new transforming gene from a chemically transformed human cell line.
    Nature. 1984 Sep 6-11;311(5981):29-33 PMID: 6590967
  32. A novel transforming gene in a human malignant melanoma cell line.
    Nature. 1984 Oct 18-24;311(5987):671-3 PMID: 6090953
  33. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.
    Blood. 1975 Mar;45(3):321-34 PMID: 163658
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-05-00
Pages
1301-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553933
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2