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PMID: 3038529 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Directed Ig class switch recombination in activated murine B cells.

The EMBO journal ·Vol. 6 ·No. 6 ·1987-06-00 ·Pages 1663-71

Winter E, Krawinkel U, Radbruch A

Abstract

Immunoglobulin class switch recombination occurs at frequencies of up to 10%/cell/generation in activated murine B-lymphocytes. We analysed cH gene rearrangements and switch recombinations from active and inactive IgH loci of B-cells activated in various ways and immortalized by cell fusion. Although about half of the IgM+ cells show rearrangement of c mu genes, the deletion of c mu is a rare event. Half of the IgG3+ and IgG1+ cells show rearrangement of c mu genes on the inactive IgH locus and the other half of the IgG+ cells have deleted c mu from both IgH loci by switch recombination. This recombination is directed to the same switch regions on both IgH loci in 60-80% of all cases. Interleukin 4 may play a critical role in programming murine B-lymphocytes for specific switch recombination.

MeSH Terms
Amino Acid Sequence Animals B-Lymphocytes/immunology Base Sequence DNA Restriction Enzymes Genes Immunoglobulin G/genetics Immunoglobulin M/genetics Immunoglobulins/genetics Lymphocyte Activation Mice Mice, Inbred Strains
Chemicals
Immunoglobulin G Immunoglobulin M Immunoglobulins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Winter E
Krawinkel U
Radbruch A
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41 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-06-00
Pages
1663-71
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553539
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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