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PMID: 3056918 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Effect of DNA sequence and structure on nuclease activity of the DexA protein of bacteriophage T4.

Journal of bacteriology ·Vol. 170 ·No. 12 ·1988-12-00 ·Pages 5830-6

Gruber H, Kern G, Gauss P, Gold L

Abstract

The bacteriophage T4 dexA gene product is required during infection of Escherichia coli strains carrying a mutation in the optA gene. We purified the DexA protein from cells which overproduced the protein. The protein was assayed for nuclease activity on synthetic di- and oligonucleotide substrates of known sequence and secondary structure. Sequence and structure significantly affected nuclease activity. The properties of the enzyme may explain the requirement for the DexA protein during infection of optA mutant hosts.

MeSH Terms
Base Sequence Escherichia coli/enzymology,genetics Exodeoxyribonuclease V Exodeoxyribonucleases/metabolism Genes Genes, Viral Kinetics Molecular Sequence Data Mutation Oligonucleotide Probes T-Phages/enzymology,genetics Viral Proteins/genetics,isolation & purification,metabolism
Chemicals
Oligonucleotide Probes Viral Proteins dexA protein, T4 phage Exodeoxyribonucleases Exodeoxyribonuclease V
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gruber H
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309.
Kern G
Gauss P
Gold L
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22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.12.5830-5836.1988
Published
1988-12-00
Pages
5830-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211689
Subset
IM
Grants
NIGMS NIH HHS · GM19963 · United States
External Links
PubMed source DOI: 10.1128/jb.170.12.5830-5836.1988 PMC Full Text View journal details
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