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PMID: 310468 Published · ppublish English Journal Article

Interdependence of the two borders in a sodium transporting epithelium. Possible regulation by the transport pool.

The Journal of membrane biology ·Vol. 40 Spec No ·1978-00-00 ·Pages 221-45

Cuthbert AW, Shum WK

Abstract

Specific binding of 14C-amiloride to the mucosal surface of frog skin epithelium (Rana temporaria) has been used as a measure of the number of sodium entry sites. All binding measurements were made with the mucosal surface bathed in a solution containing 1.1 mM sodium. When manipulations were used which increased the intracellular concentration of sodium the amount of amiloride bound was reduced. The manipulations included flushing the mucosal surface with solutions containing 111 mM sodium after serosal efflux was inhibited with ouabain or potassium removal. Similar results were obtained when cells were loaded with lithium. These effects on amiloride binding did not appear to depend on changes in membrane potential or upon changes in affinity of amiloride for its binding site. It appears that inhibition of serosal sodium efflux from the epithelium causes a reduction of mucosal sodium influx by making entry sites unavailable. This latter may be a result, directly or indirectly, of the sodium concentration in the sodium transport pool.

MeSH Terms
Amiloride/metabolism,pharmacology Animals Anura Binding, Competitive Biological Transport, Active Epithelium/metabolism In Vitro Techniques Mathematics Mucous Membrane/metabolism Rana temporaria Skin/metabolism Sodium/metabolism
Chemicals
Amiloride Sodium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cuthbert A W
Shum W K
References (37)
37 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF02026008
Published
1978-00-00
Pages
221-45
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02026008 View journal details
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