Home LiteratureArticle Details
PMID: 3118797 Published · ppublish English Journal Article

Location of antibiotic resistance markers in clinical isolates of Enterococcus faecalis with similar antibiotypes.

Antimicrobial agents and chemotherapy ·Vol. 31 ·No. 9 ·1987-09-00 ·Pages 1394-402

Pepper K, Horaud T, Le Bouguénec C, de Cespédès G

Abstract

Eight wild-type strains of Enterococcus faecalis, resistant to chloramphenicol (Cmr), erythromycin (Emr), tetracycline (Tcr), and minocycline (Mnr), were examined for the genetic basis of their antibiotic resistance, Five of the strains transferred all of their antibiotic resistance markers by conjugation, while the other three strains transferred only Tcr and Mnr. Cmr and Emr determinants were localized by DNA-DNA hybridization experiments, in which the Cmr gene of plasmid pIP501, of group B Streptococcus origin, and the Emr gene of transposon Tn917, of E. faecalis origin, served as probes. A chromosomal location was found for the nonconjugative Cmr and Emr markers of one wild-type strain. In two strains these markers were carried by nonconjugative plasmids, and in the other strains they were carried by plasmids that transferred by conjugation. Plasmids isolated from three transconjugants resistant to tetracycline but susceptible to minocycline bore nucleotide sequences homologous to the tetL gene. Nucleotide sequences homologous to conjugative transposon Tn916, of E. faecalis origin, were detected by hybridization in the tetracycline-minocycline-resistant transconjugants. Three of these transconjugants were plasmid free, while four harbored conjugative cryptic plasmids. Sequences homologous to Tn916 were also found on two conjugative plasmids, one of which appeared to be a conjugative cryptic plasmid that had acquired chromosomal Tcr Mnr markers during transfer.

MeSH Terms
Chloramphenicol Resistance Chromosomes, Bacterial Conjugation, Genetic Drug Resistance, Microbial Enterococcus faecalis/drug effects,genetics Erythromycin/pharmacology Genes, Bacterial Minocycline/pharmacology Nucleic Acid Hybridization R Factors Sequence Homology, Nucleic Acid Streptomycin/pharmacology Tetracycline Resistance
Chemicals
Erythromycin Minocycline Streptomycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pepper K
Laboratoire des Staphylocoques et des Streptocoques, Institut Pasteur, Paris, France.
Horaud T
Le Bouguénec C
de Cespédès G
References (39)
39 references, click to expand
  1. Plasmid-linked tetracycline and erythromycin resistance in group D "streptococcus".
    Ann Inst Pasteur (Paris). 1972 Dec;123(6):755-9 PMID: 4347774
  2. Isolation of plasmid deletion Mutants and study of their instability.
    Plasmid. 1981 Sep;6(2):193-201 PMID: 7029580
  3. Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes.
    J Bacteriol. 1974 Feb;117(2):360-72 PMID: 4204433
  4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  5. R plasmids in Streptococcus agalactiae (group B).
    Antimicrob Agents Chemother. 1976 Nov;10(5):795-801 PMID: 795369
  6. Elimination of plasmids from several bacterial species by novobiocin.
    Antimicrob Agents Chemother. 1977 Sep;12(3):423-6 PMID: 907332
  7. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  8. Improved lysis of group N streptococci for isolation and rapid characterization of plasmid deoxyribonucleic acid.
    Appl Environ Microbiol. 1978 Mar;35(3):592-600 PMID: 416755
  9. Induced cell aggregation and mating in Streptococcus faecalis: evidence for a bacterial sex pheromone.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3479-83 PMID: 98769
  10. Conjugative R plasmids in Streptococcus agalactiae (group B).
    Plasmid. 1979 Apr;2(2):197-206 PMID: 109871
  11. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  12. Properties of erythromycin-inducible transposon Tn917 in Streptococcus faecalis.
    J Bacteriol. 1980 Mar;141(3):1366-74 PMID: 6245068
  13. Identification of tetracycline-resistant R-plasmids in Streptococcus agalactiae (group B).
    Antimicrob Agents Chemother. 1980 Nov;18(5):753-60 PMID: 7004347
  14. [Group D streptococci in human infections: identification and sensitivity to antibiotics (author's transl)].
    Ann Microbiol (Paris). 1980 Sep-Oct;131B(2):131-44 PMID: 7458116
  15. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of "conjugal" transfer in the absence of a conjugative plasmid.
    J Bacteriol. 1981 Jan;145(1):494-502 PMID: 6257641
  16. Plasmids, drug resistance, and gene transfer in the genus Streptococcus.
    Microbiol Rev. 1981 Sep;45(3):409-36 PMID: 6272080
  17. Heterogeneity of tetracycline resistance determinants in Streptococcus.
    J Bacteriol. 1982 Mar;149(3):995-1004 PMID: 6801018
  18. Location of antibiotic resistance determinants, copy control, and replication functions on the double-selective streptococcal cloning vector pGB301.
    Mol Gen Genet. 1981;184(1):115-20 PMID: 6278245
  19. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance.
    J Bacteriol. 1982 May;150(2):815-25 PMID: 6950931
  20. Characterization of two tetracycline resistance determinants in Streptococcus faecalis JH1.
    J Bacteriol. 1982 May;150(2):835-43 PMID: 6802800
  21. Conjugative R plasmids in Streptococcus faecium (group D).
    Antimicrob Agents Chemother. 1982 May;21(5):698-705 PMID: 6285813
  22. Mapping of Streptococcus faecalis plasmids pAD1 and pAD2 and studies relating to transposition of Tn917.
    J Bacteriol. 1982 Dec;152(3):1220-30 PMID: 6292164
  23. Narrow host range of some streptococcal R plasmids.
    Plasmid. 1982 Sep;8(2):199-206 PMID: 7178293
  24. Streptococcus plasmid pAM alpha 1 is a composite of two separable replicons, one of which is closely related to Bacillus plasmid pBC16.
    J Bacteriol. 1983 Aug;155(2):607-15 PMID: 6307973
  25. Streptococcus faecalis R plasmid pJH1 contains an erythromycin resistance transposon (Tn3871) similar to transposon Tn917.
    J Bacteriol. 1984 Jun;158(3):1172-4 PMID: 6327631
  26. Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning of genes from gram-positive bacteria.
    J Bacteriol. 1984 Jul;159(1):214-21 PMID: 6330031
  27. Translocation of antibiotic resistance markers of a plasmid-free Streptococcus pyogenes (group A) strain into different streptococcal hemolysin plasmids.
    Mol Gen Genet. 1984;194(3):377-87 PMID: 6330497
  28. Chloramphenicol acetyltransferase gene of staphylococcal plasmid pC221. Nucleotide sequence analysis and expression studies.
    FEBS Lett. 1985 Jan 1;179(1):101-6 PMID: 3855295
  29. A conjugative transposon (Tn919) in Streptococcus sanguis.
    Infect Immun. 1985 Feb;47(2):415-20 PMID: 2981772
  30. Streptococcus faecalis sex pheromone (cAM373) also produced by Staphylococcus aureus and identification of a conjugative transposon (Tn918).
    J Bacteriol. 1985 Jun;162(3):1212-20 PMID: 2987186
  31. Evidence for a disseminated erythromycin resistance determinant mediated by Tn917-like sequences among group D streptococci isolated from pigs, chickens, and humans.
    Antimicrob Agents Chemother. 1985 Apr;27(4):439-44 PMID: 2988428
  32. Molecular genetics of resistance to macrolides, lincosamides and streptogramin B (MLS) in streptococci.
    J Antimicrob Chemother. 1985 Jul;16 Suppl A:111-35 PMID: 3932298
  33. Complete nucleotide sequence of macrolide-lincosamide-streptogramin B-resistance transposon Tn917 in Streptococcus faecalis.
    J Bacteriol. 1985 Nov;164(2):782-96 PMID: 2997130
  34. Streptococcal tetracycline resistance mediated at the level of protein synthesis.
    J Bacteriol. 1986 Feb;165(2):564-9 PMID: 3080409
  35. Identification of regions of the Streptococcus faecalis plasmid pCF-10 that encode antibiotic resistance and pheromone response functions.
    Plasmid. 1986 May;15(3):230-41 PMID: 3012615
  36. Conjugative transposons and the dissemination of antibiotic resistance in streptococci.
    Annu Rev Microbiol. 1986;40:635-59 PMID: 3022640
  37. Dispersal of a plasmid-borne chloramphenicol resistance gene in streptococcal and enterococcal plasmids.
    Plasmid. 1986 Nov;16(3):195-203 PMID: 3101076
  38. Enterococcus faecalis hemolysin-bacteriocin plasmids belong to the same incompatibility group.
    Appl Environ Microbiol. 1987 Mar;53(3):567-70 PMID: 3107464
  39. Characterization of three plasmid deoxyribonucleic acid molecules in a strain of Streptococcus faecalis: identification of a plasmid determining erythromycin resistance.
    J Bacteriol. 1974 Jan;117(1):283-9 PMID: 4202995
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.31.9.1394
Published
1987-09-00
Pages
1394-402
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC174949
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.31.9.1394 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2