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PMID: 3141902 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sigma region located between C mu and C delta genes of human immunoglobulin heavy chain: possible involvement of tRNA-like structure in RNA splicing.

Nucleic acids research ·Vol. 16 ·No. 20 ·1988-10-25 ·Pages 9497-511

Akahori Y, Handa H, Imai K, Abe M, Kameyama K, Hibiya M, Yasui H, Okamura K, Naito M, Matsuoka H

Abstract

Noncoding regions within the cluster of immunoglobulin heavy chain constant genes in the human genome contained a number of repeats. In the mu-delta intron, two repeating units were contained. One 442-base-long fragment located JH-mu intron (defined as "sigma mu(sigma mu)") occupied the position in the mu-delta intron. The other 1166-base-long fragment located somewhere in front of S (class switch) region of C gamma gene was also found in the mu-delta intron. We defined the repeats in the mu-delta intron as "SIGMA (sigma)". The polarities of the longer repeats in the genome were opposite between the mu-delta intron and the upstreams of C gamma genes. These inverted copies (defined as sigma gamma 3 and sigma gamma 4), located 6 kb upstream of their respective C gamma's, were apparently transcribed in vitro, via RNA polymerase III and transcripts should have contained tRNA-like structures. Small DNA fragments capable of encoding tRNA-like structures were also found in corresponding regions of mouse Ig C gamma cluster.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA Genes, Immunoglobulin Humans Immunoglobulin Constant Regions/genetics Immunoglobulin Heavy Chains/genetics Introns Mice Molecular Sequence Data Nucleic Acid Conformation Promoter Regions, Genetic RNA Polymerase III RNA Splicing RNA, Transfer/genetics Sequence Homology, Nucleic Acid
Chemicals
Immunoglobulin Constant Regions Immunoglobulin Heavy Chains DNA RNA, Transfer RNA Polymerase III
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Akahori Y
Institute for Comprehensive Medical Science, Fujita-Gakuen Health University, Aichi, Japan.
Handa H
Imai K
Abe M
Kameyama K
Hibiya M
Yasui H
Okamura K
Naito M
Matsuoka H
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.20.9497
Published
1988-10-25
Pages
9497-511
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338759
Subset
IM
Databases
GENBANK
X12842, X12843
External Links
PubMed source DOI: 10.1093/nar/16.20.9497 PMC Full Text View journal details
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