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PMID: 6089118 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The sequence of the human immunoglobulin mu-delta intron reveals possible vestigial switch segments.

Nucleic acids research ·Vol. 12 ·No. 16 ·1984-08-24 ·Pages 6523-35

Milstein CP, Deverson EV, Rabbitts TH

Abstract

We present the full sequence of an insert of a lambda phage clone which contains a segment of human DNA stretching from the secreted mu(mu s) constant region gene through to the beginning of the constant region gene and including the membrane mu(mu m) segments. The segment of 8.6kb extending from mu s to the first constant domain of delta(C delta 1) has been completely sequenced and reveals little conservation in comparison to the corresponding mouse sequence. The outstanding feature of the mu s-mu m intron is the occurrence of a potential Z-DNA forming region situated at 285bp downstream of the mu s poly A addition signal. A similar DNA stretch exists in mouse and may represent a site for transcriptional control of mu gene expression. The mu m-C delta 1 intron is much longer (6Kb) than the corresponding mouse intron and includes a series of different repeats, which start at 430bp downstream of the mu m poly A addition site and continue for 3.5Kb, ending about 1.5Kb from the beginning of C delta 1. This series of repeats may be a vestigial switch sequence used in the production of the secreting cells which are the progenitors of the rare human IgD myelomas.

MeSH Terms
Bacteriophage lambda/genetics Base Sequence Cloning, Molecular Computers DNA/genetics DNA Restriction Enzymes Genes Humans Immunoglobulin Heavy Chains/genetics Immunoglobulin delta-Chains/genetics Immunoglobulin mu-Chains/genetics Nucleic Acid Conformation Transcription, Genetic
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin delta-Chains Immunoglobulin mu-Chains DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Milstein C P
Deverson E V
Rabbitts T H
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48 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.16.6523
Published
1984-08-24
Pages
6523-35
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320093
Subset
IM
Databases
GENBANK
X00799
External Links
PubMed source DOI: 10.1093/nar/12.16.6523 PMC Full Text View journal details
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