Home LiteratureArticle Details
PMID: 3142848 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of the tetracycline resistance gene of plasmid pT181 of Staphylococcus aureus.

Journal of bacteriology ·Vol. 170 ·No. 12 ·1988-12-00 ·Pages 5522-8

Mojumdar M, Khan SA

Abstract

Some genetic and biochemical properties of the tetracycline resistance element of the Staphylococcus aureus plasmid pT181 have been studied. Resequencing of a portion of the tetracycline resistance gene (tet) showed the presence of a single open reading frame of 1,299 nucleotides capable of encoding a polypeptide of 433 amino acids. Analysis of BAL 31 nuclease-generated deletion mutants of the tet gene showed the presence of two complementation groups within this region. Northern blot hybridizations demonstrated that the tet gene encodes a single mRNA, and its initiation site has been mapped by S1 nuclease protection experiments. We also identified an approximately 52,000-dalton tetracycline-inducible polypeptide in Bacillus subtilis minicells carrying pT181. Induction of the tet gene by tetracycline resulted in a 4-fold increase in the levels of TET mRNA and at least a 15-fold increase in the amount of TET protein in B. subtilis minicells.

MeSH Terms
Bacillus subtilis/genetics Base Sequence Genes, Bacterial Genetic Complementation Test Molecular Sequence Data Mutation R Factors RNA, Messenger/genetics Restriction Mapping Staphylococcus aureus/genetics Tetracycline Resistance/genetics Transcription, Genetic
Chemicals
RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mojumdar M
Department of Microbiology, School of Medicine, University of Pittsburgh, Pennsylvania 15261.
Khan S A
References (45)
45 references, click to expand
  1. Post-transcriptional regulation of chloramphenicol acetyl transferase.
    J Bacteriol. 1984 May;158(2):543-50 PMID: 6202672
  2. Biochemical and genetic characterization of the tet determinant of Bacillus plasmid pAB124.
    J Bacteriol. 1984 Apr;158(1):134-40 PMID: 6325384
  3. Control of pT181 replication I. The pT181 copy control function acts by inhibiting the synthesis of a replication protein.
    EMBO J. 1984 Oct;3(10):2399-405 PMID: 6499834
  4. Control of pT181 replication II. Mutational analysis.
    EMBO J. 1984 Oct;3(10):2407-14 PMID: 6437809
  5. Plasmid pT181 replication is regulated by two countertranscripts.
    Proc Natl Acad Sci U S A. 1985 Feb;82(3):638-42 PMID: 2579377
  6. Nucleotide sequence of ermA, a macrolide-lincosamide-streptogramin B determinant in Staphylococcus aureus.
    J Bacteriol. 1985 May;162(2):633-40 PMID: 2985541
  7. Antibiotic resistance resulting from decreased drug accumulation.
    Br Med Bull. 1984 Jan;40(1):11-7 PMID: 6442873
  8. Nucleotide sequence of the tetracycline resistance gene of pTHT15, a thermophilic Bacillus plasmid: comparison with staphylococcal TcR controls.
    Gene. 1985;37(1-3):131-8 PMID: 2996983
  9. Chloramphenicol-induced translation of cat-86 mRNA requires two cis-acting regulatory regions.
    J Bacteriol. 1985 Nov;164(2):696-703 PMID: 2414270
  10. Streptococcal tetracycline resistance mediated at the level of protein synthesis.
    J Bacteriol. 1986 Feb;165(2):564-9 PMID: 3080409
  11. The nucleotide sequence of the tetracycline resistance gene of plasmid pNS1981 from Bacillus subtilis differs from pTHT15 from a Thermophilic bacillus by two base pairs.
    Plasmid. 1986 Jul;16(1):72-3 PMID: 3090576
  12. Transcription termination and the regulation of gene expression.
    Annu Rev Biochem. 1986;55:339-72 PMID: 3527045
  13. Demonstration of erythromycin-dependent stalling of ribosomes on the ermC leader transcript.
    J Biol Chem. 1987 Feb 5;262(4):1766-71 PMID: 3027099
  14. Heterogeneity of tetracycline resistance determinants.
    Plasmid. 1980 Mar;3(2):99-108 PMID: 6927764
  15. Energy-dependent efflux mediated by class L (tetL) tetracycline resistance determinant from streptococci.
    Antimicrob Agents Chemother. 1987 Oct;31(10):1648-50 PMID: 3324958
  16. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  17. Studies on plasmid replication. I. Plasmid incompatibility and establishment in Staphylococcus aureus.
    J Mol Biol. 1972 Jul 21;68(2):285-302 PMID: 4262657
  18. Analysis of bacteriophage T7 early RNAs and proteins on slab gels.
    J Mol Biol. 1973 Sep 15;79(2):237-48 PMID: 4760132
  19. Detection of an inducible membrane protein associated with R-factor-mediated tetracycline resistance.
    Biochem Biophys Res Commun. 1974 Feb 27;56(4):1060-8 PMID: 4596957
  20. Synthesis of an R plasmid protein associated with tetracycline resistance is negatively regulated.
    Proc Natl Acad Sci U S A. 1976 May;73(5):1509-12 PMID: 775491
  21. Three distinct plasmids originating in the same Staphylococcus aureus strain.
    Arch Roum Pathol Exp Microbiol. 1976 Jan-Jun;35(1-2):111-8 PMID: 1008685
  22. Ribosome-tetracycline interactions.
    Biochemistry. 1977 Sep 6;16(18):4133-8 PMID: 334241
  23. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  24. On the nature of tetracycline resistance controlled by the plasmid pSC101.
    Cell. 1978 Jan;13(1):73-81 PMID: 340050
  25. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  26. Bacterial resistance to the tetracyclines.
    Microbiol Rev. 1978 Dec;42(4):707-24 PMID: 739968
  27. Tn554--a site-specific repressor-controlled transposon in Staphylococcus aureus.
    Nature. 1979 Mar 29;278(5703):476-8 PMID: 156306
  28. Studies on the synthesis of plasmid-coded proteins and their control in Bacillus subtilis minicells.
    Plasmid. 1979 Apr;2(2):279-89 PMID: 109873
  29. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  30. Molecular cloning of the DNA ligase gene from bacteriophage T4. II. Amplification and preparation of the gene product.
    J Mol Biol. 1979 Aug 15;132(3):493-505 PMID: 392109
  31. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  32. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  33. Posttranscriptional regulation of an erythromycin resistance protein specified by plasmic pE194.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3903-7 PMID: 6159624
  34. Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3974-7 PMID: 7001450
  35. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  36. Conformational alteration of mRNA structure and the posttranscriptional regulation of erythromycin-induced drug resistance.
    Nucleic Acids Res. 1980 Dec 20;8(24):6081-97 PMID: 6162157
  37. Tetracycline resistance genes from Bacillus plasmid pAB124 confer decreased accumulation of the antibiotic in Bacillus subtilis but not in Escherichia coli.
    J Bacteriol. 1981 Mar;145(3):1417-20 PMID: 6782092
  38. Identification of the tetracycline resistance promoter and repressor in transposon Tn10.
    J Bacteriol. 1981 Aug;147(2):297-304 PMID: 6267006
  39. Heterogeneity of tetracycline resistance determinants in Streptococcus.
    J Bacteriol. 1982 Mar;149(3):995-1004 PMID: 6801018
  40. Coding sequence for the pT181 repC product: a plasmid-coded protein uniquely required for replication.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4108-12 PMID: 6287465
  41. Tn1721-encoded tetracycline resistance: mapping of structural and regulatory genes mediating resistance.
    J Bacteriol. 1983 Jan;153(1):116-23 PMID: 6294046
  42. Analysis of tetracycline resistance encoded by transposon Tn10: deletion mapping of tetracycline-sensitive point mutations and identification of two structural genes.
    J Bacteriol. 1983 Feb;153(2):921-9 PMID: 6296060
  43. Intracistronic complementation of the tetracycline resistance membrane protein of Tn10.
    J Bacteriol. 1984 Jan;157(1):211-7 PMID: 6317652
  44. Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid from Staphylococcus aureus.
    Plasmid. 1983 Nov;10(3):251-9 PMID: 6657777
  45. Regulatory regions that control expression of two chloramphenicol-inducible cat genes cloned in Bacillus subtilis.
    J Bacteriol. 1984 Jun;158(3):784-90 PMID: 6327638
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.12.5522-5528.1988
Published
1988-12-00
Pages
5522-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211646
Subset
IM
Grants
NIGMS NIH HHS · GM-31685 · United States
Databases
GENBANK
M23644
External Links
PubMed source DOI: 10.1128/jb.170.12.5522-5528.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2