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PMID: 3160662 Published · ppublish English Journal Article

Cloning and expression of two Streptococcus mutans glucosyltransferases in Escherichia coli K-12.

Infection and immunity ·Vol. 49 ·No. 2 ·1985-08-00 ·Pages 414-6

Gilpin ML, Russell RR, Morrissey P

Abstract

Chromosomal DNA from Streptococcus mutans strain MFe28 (serotype h) was cloned in the bacteriophage vector lambda L47.1. Two classes of recombinants were found which expressed glucosyltransferase activity in phage plaques: (i) gtfS, which expressed a glucosyltransferase synthesizing a water-soluble, dextranase-sensitive glucan, and (ii) gtfI, which expressed a primer-dependent glucosyltransferase synthesizing an insoluble glucan.

MeSH Terms
Bacteriophage lambda/genetics Cloning, Molecular DNA, Recombinant/metabolism Escherichia coli/enzymology,genetics Genes Genes, Bacterial Glucosyltransferases/genetics Molecular Weight Streptococcus mutans/enzymology,genetics
Chemicals
DNA, Recombinant Glucosyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gilpin M L
Russell R R
Morrissey P
References (20)
20 references, click to expand
  1. Bacterial adherence in oral microbial ecology.
    Annu Rev Microbiol. 1975;29:19-44 PMID: 1180512
  2. Expression of a gene for glucan-binding protein from Streptococcus mutans in Escherichia coli.
    J Gen Microbiol. 1985 Feb;131(2):295-9 PMID: 3156960
  3. Purification, resolution, and interaction of the glucosyltransferases of Streptococcus mutans 6715.
    Infect Immun. 1977 Oct;18(1):237-46 PMID: 908619
  4. Use of triton X-100 to overcome the inhibition of fructosyltransferase by SDS.
    Anal Biochem. 1979 Aug;97(1):173-5 PMID: 484838
  5. Glycosyltransferases of Streptococcus mutans strain Ingbritt.
    Microbios. 1978;23(93-94):136-46 PMID: 117283
  6. Biology, immunology, and cariogenicity of Streptococcus mutans.
    Microbiol Rev. 1980 Jun;44(2):331-84 PMID: 6446023
  7. A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes.
    Gene. 1980 Aug;10(3):249-59 PMID: 6254843
  8. Resolution of Streptococcus mutans glycosyltransferases into two components essential to water-insoluble glucan synthesis.
    FEBS Lett. 1981 Jun 15;128(2):213-6 PMID: 6455304
  9. The isolation of characterization of Streptococcus mutans serotype h from dental plaque of monkeys (Macaca fascicularis).
    J Gen Microbiol. 1981 Jun;124(2):271-9 PMID: 6460088
  10. Purification and properties of Streptococcus mutans extracellular glucosyltransferase.
    Biochim Biophys Acta. 1982 Mar 18;702(1):72-80 PMID: 6461359
  11. Purification and properties of glucosyltransferase responsible for water-insoluble glucan synthesis from Streptococcus mutans.
    Infect Immun. 1982 Jul;37(1):1-9 PMID: 6179873
  12. Expression of Streptococcus mutans aspartate-semialdehyde dehydrogenase gene cloned into plasmid pBR322.
    J Gen Microbiol. 1982 May;128(5):1135-45 PMID: 6125561
  13. Expression of a Streptococcus mutans glucosyltransferase gene in Escherichia coli.
    J Bacteriol. 1983 Jan;153(1):211-21 PMID: 6217191
  14. Mechanism of synthesis of D-glucans by D-glucosyltransferases from Streptococcus mutans 6715.
    Carbohydr Res. 1983 Mar 1;113(2):301-15 PMID: 6220802
  15. Production of monoclonal antibody against a glucosyltransferase of Streptococcus mutans 6715.
    Infect Immun. 1983 Aug;41(2):872-5 PMID: 6223885
  16. Cloning, expression, and mapping of the Staphylococcus aureus alpha-hemolysin determinant in Escherichia coli K-12.
    Infect Immun. 1983 Sep;41(3):1105-11 PMID: 6350179
  17. Comparative study of Streptococcus mutans extracellular glycosyltransferases by isoelectric focusing.
    J Gen Microbiol. 1983 Oct;129(10):3261-9 PMID: 6228639
  18. The origin and composition of multiple forms of dextransucrase from Streptococcus sanguis.
    Biochim Biophys Acta. 1984 Apr 27;786(1-2):42-8 PMID: 6231957
  19. Immunochemical characteristics of Streptococcus mutans serotype h carbohydrate antigen.
    Microbiol Immunol. 1984;28(4):407-13 PMID: 6748966
  20. Characterization of extracellular glucosyltransferase activity of Steptococcus mutans.
    Infect Immun. 1975 Oct;12(4):738-49 PMID: 1193714
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.49.2.414-416.1985
Published
1985-08-00
Pages
414-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC262032
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.49.2.414-416.1985 PMC Full Text View journal details
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