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PMID: 3275614 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structure and transcription of the allantoate permease gene (DAL5) from Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 170 ·No. 1 ·1988-01-00 ·Pages 266-71

Rai R, Genbauffe FS, Cooper TG

Abstract

We determined the nucleotide sequence of the DAL5 gene, which encodes a component of the allantoate transport system. Translation of the sequence revealed that the DAL5 gene product is highly hydrophobic. It possesses an alternating motif of hydrophilic sequences that can potentially be folded into alpha-helices and hydrophobic sequences that can potentially be folded into beta-pleated sheets. These are expected characteristics of an integral membrane protein, which correlate well with DAL5 gene function. S1 protection fragments generated by DAL5 transcripts exhibited high heterogeneity over a 30-base-pair range. This pattern of fragments was not affected by growth conditions of the cells or the conditions of the assay.

MeSH Terms
Allantoin/metabolism Amino Acid Sequence Base Sequence DNA, Fungal/genetics Gene Expression Regulation Genes, Fungal Membrane Transport Proteins/genetics Molecular Sequence Data Nucleic Acid Hybridization RNA, Fungal/genetics Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Transcription, Genetic
Chemicals
DAL4 protein, S cerevisiae DAL5 protein, S cerevisiae DNA, Fungal Membrane Transport Proteins RNA, Fungal Saccharomyces cerevisiae Proteins Allantoin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rai R
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
Genbauffe F S
Cooper T G
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.1.266-271.1988
Published
1988-01-00
Pages
266-71
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210637
Subset
IM
Grants
NIGMS NIH HHS · R01 GM035642 · United States
NIGMS NIH HHS · GM-35642 · United States
Databases
GENBANK
M24098
External Links
PubMed source DOI: 10.1128/jb.170.1.266-271.1988 PMC Full Text View journal details
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