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PMID: 3290192 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Ancient heat shock gene is dispensable.

Journal of bacteriology ·Vol. 170 ·No. 7 ·1988-07-00 ·Pages 2977-83

Bardwell JC, Craig EA

Abstract

Hsp83 is a major eucaryotic heat shock protein and one of the most conserved proteins known. We have isolated an Escherichia coli gene homologous to eucaryotic Hsp83 and used it to construct a deletion mutation. The E. coli mutant was viable but had a slight growth disadvantage that increased with temperature.

MeSH Terms
Chromosome Deletion DNA, Bacterial/genetics Escherichia coli/genetics,growth & development Heat-Shock Proteins/biosynthesis,genetics Hot Temperature Mutation Nucleic Acid Hybridization Plasmids
Chemicals
DNA, Bacterial Heat-Shock Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bardwell J C
Department of Physiological Chemistry, University of Wisconsin Medical School, Madison 53706.
Craig E A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.7.2977-2983.1988
Published
1988-07-00
Pages
2977-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211237
Subset
IM
Grants
NIGMS NIH HHS · GM72870 · United States
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/jb.170.7.2977-2983.1988 PMC Full Text View journal details
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