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PMID: 3327691 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Mutants of elongation factor Tu promote ribosomal frameshifting and nonsense readthrough.

The EMBO journal ·Vol. 6 ·No. 13 ·1987-12-20 ·Pages 4235-9

Hughes D, Atkins JF, Thompson S

Abstract

This is the first report of ribosomal frameshifting promoted by mutants of the elongation factor Tu (EF-Tu). EF-Tu mutants can suppress both -1 and +1 frameshift mutations. The level of nonsense readthrough is also increased at some UGA (this paper) and UAG (Hughes, 1987) sites by these mutants. Suppression occurs when a mutant tuf allele is paired with a wild-type copy of the other tuf gene but is most efficient when both tuf genes are mutant. Frameshifting mediated by the tuf alleles studied, tufA8 and tufB103, is not general; indeed most frameshift mutations are not suppressed. Several possible mechanisms by which mutant EF-Tu may cause frameshifting are discussed.

MeSH Terms
Alleles Base Sequence Codon Escherichia coli/genetics,metabolism Genes Genes, Bacterial Molecular Sequence Data Mutation Operon Peptide Elongation Factor Tu/genetics Ribosomes/metabolism Salmonella Phages/genetics Salmonella typhimurium/genetics,metabolism
Chemicals
Codon Peptide Elongation Factor Tu
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hughes D
Department of Genetics, Trinity College, Dublin Ireland.
Atkins J F
Thompson S
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37 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-12-20
Pages
4235-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553909
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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