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PMID: 3335481 Published · ppublish English Journal Article

Gene organization and structure of the Streptomyces lividans gal operon.

Journal of bacteriology ·Vol. 170 ·No. 1 ·1988-01-00 ·Pages 203-12

Adams CW, Fornwald JA, Schmidt FJ, Rosenberg M, Brawner ME

Abstract

We present the gene organization and DNA sequence of the Streptomyces lividans galactose utilization genes. Complementation of Escherichia coli galE, galT, or galK mutants and DNA sequence analysis were used to demonstrate that the galactose utilization genes are organized within an operon with the gene order galT, galE, and galK. Comparison of the inferred protein sequences for the S. lividans gal gene products to the corresponding E. coli and Saccharomyces carlbergensis sequences identified regions of structural homology within each of the galactose utilization enzymes. Finally, we discuss a potential relationship between the gene organization of the operon and the functional roles of the gal enzymes in cellular metabolism.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Bacterial/genetics Galactokinase/genetics Galactose/metabolism Genes, Bacterial Genetic Complementation Test Molecular Sequence Data Nucleic Acid Hybridization Operon Streptomyces/enzymology,genetics,metabolism
Chemicals
DNA, Bacterial Galactokinase Galactose
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Adams C W
Molecular Genetics Department, SmithKline and French Laboratories, King of Prussia, Pennsylvania 19406-0939.
Fornwald J A
Schmidt F J
Rosenberg M
Brawner M E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.1.203-212.1988
Published
1988-01-00
Pages
203-12
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210627
Subset
IM
Databases
GENBANK
M18953
External Links
PubMed source DOI: 10.1128/jb.170.1.203-212.1988 PMC Full Text View journal details
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