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PMID: 6715281 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sequence of the Saccharomyces GAL region and its transcription in vivo.

Journal of bacteriology ·Vol. 158 ·No. 1 ·1984-04-00 ·Pages 269-78

Citron BA, Donelson JE

Abstract

In Saccharomyces, the enzymes used to convert galactose to glucose are specified by three coordinately expressed, tightly linked genes, GAL7, GAL10, and GAL1. These genes are induced by galactose and are controlled by the positive regulator gene gal4 and the negative regulator gene gal80. GAL81 mutations, which are known to alter the gal4 protein, produce a constitutive phenotype. We have cloned fragments of Saccharomyces carlsbergensis DNA that span 26.3 kilobases surrounding the three clustered GAL genes. About 5 kilobases of the sequence was determined, which includes the entire GAL1 gene, the two intercistronic regions, and portions of the coding sequences of GAL10 and GAL7. Some amino acid homology between the GAL1 gene product, galactokinase, and the Escherichia coli galactokinase was detected. By using various Saccharomyces DNA fragments, the accumulation of GAL1 and GAL10 RNA in yeast cells after induction with galactose was studied. Our results, using wild-type, gal4-, gal80-, and GAL81-1- yeast cells, support the hypothesis that control is exerted at the transcriptional level.

MeSH Terms
Base Sequence Cloning, Molecular Codon Galactokinase/genetics Galactose/metabolism Genes Genes, Fungal Genes, Regulator Kinetics Operon RNA, Fungal/biosynthesis,genetics Saccharomyces/enzymology,genetics,metabolism Transcription, Genetic UDPglucose 4-Epimerase/genetics UTP-Hexose-1-Phosphate Uridylyltransferase/genetics
Chemicals
Codon RNA, Fungal Galactokinase UTP-Hexose-1-Phosphate Uridylyltransferase UDPglucose 4-Epimerase Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Citron B A
Donelson J E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.158.1.269-278.1984
Published
1984-04-00
Pages
269-78
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215408
Subset
IM
Grants
NIGMS NIH HHS · GM 07091 · United States
Databases
GENBANK
K01609, K01752, K01753
External Links
PubMed source DOI: 10.1128/jb.158.1.269-278.1984 PMC Full Text View journal details
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