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PMID: 3470599 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human recipient cell for oncogene transfection studies.

Molecular and cellular biology ·Vol. 7 ·No. 3 ·1987-03-00 ·Pages 1280-4

Tainsky MA, Shamanski FL, Blair D, Vande Woude G

Abstract

We used human oncogene DNA to transform the nontumorigenic, revertant, human osteosarcoma cell line HOS TE-85 clone 5 (ATCC CRL 1543) to tumorigenicity in athymic nude mice with latency periods as short as 3 weeks. These cells were also transformed by genetic markers in genomic DNA samples. Because of their low rate of spontaneous tumor formation and the simplicity of culturing them, HOS cells provide a human cell alternative to NIH 3T3 murine fibroblasts for oncogene transfection studies.

MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic DNA/genetics Humans Mice Mice, Nude Neoplasm Transplantation Oncogenes Osteosarcoma/genetics Proto-Oncogenes Transfection
Chemicals
DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tainsky M A
Shamanski F L
Blair D
Vande Woude G
References (19)
19 references, click to expand
  1. Cultivation in vitro of cells derived from a human osteosarcoma.
    Cancer. 1971 Feb;27(2):397-402 PMID: 5100401
  2. Transformation of human cells by DNAs ineffective in transformation of NIH 3T3 cells.
    Proc Natl Acad Sci U S A. 1985 Apr;82(8):2399-403 PMID: 3857589
  3. Transformation of human osteosarcoma cells by a chemical carcinogen.
    J Natl Cancer Inst. 1975 Dec;55(6):1291-4 PMID: 813009
  4. Cloning of integrated Moloney sarcoma proviral DNA sequences in bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4464-8 PMID: 159456
  5. Analysis of the virogenes related to the rhesus monkey endogenous type C retrovirus in monkeys and apes.
    J Virol. 1981 Mar;37(3):922-30 PMID: 6262537
  6. Molecular cloning of avian myelocytomatosis virus (MC29) transforming sequences.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1518-22 PMID: 6262810
  7. Abelson murine leukemia virus: molecular cloning of infectious integrated proviral DNA.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2077-81 PMID: 6264461
  8. T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes.
    Nature. 1982 Jul 22;298(5872):343-7 PMID: 6283384
  9. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  10. New method for detecting cellular transforming genes.
    Science. 1982 Dec 10;218(4577):1122-5 PMID: 6293052
  11. Isolation and preliminary characterization of the transforming gene of a human neuroblastoma cell line.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):383-7 PMID: 6300838
  12. Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes.
    Nature. 1983 Aug 18-24;304(5927):596-602 PMID: 6308472
  13. Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture.
    Nature. 1983 Aug 18-24;304(5927):602-6 PMID: 6308473
  14. Characterization of human transforming genes from chemically transformed, teratocarcinoma, and pancreatic carcinoma cell lines.
    Cancer Res. 1984 Jan;44(1):1-10 PMID: 6418375
  15. Resistance of human cells to tumorigenesis induced by cloned transforming genes.
    Proc Natl Acad Sci U S A. 1983 Dec;80(24):7601-5 PMID: 6324180
  16. Transformation of human cells by DNA transfection.
    Cancer Res. 1984 Jul;44(7):2769-72 PMID: 6586290
  17. An activated rasN gene: detected in late but not early passage human PA1 teratocarcinoma cells.
    Science. 1984 Aug 10;225(4662):643-5 PMID: 6740333
  18. New human transforming genes detected by a tumorigenicity assay.
    Mol Cell Biol. 1984 Sep;4(9):1695-705 PMID: 6092933
  19. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.3.1280-1284.1987
Published
1987-03-00
Pages
1280-4
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365204
Subset
IM
Grants
NCI NIH HHS · P30 CA-16672 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.3.1280-1284.1987 PMC Full Text View journal details
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