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PMID: 3524454 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A simple and generally applicable procedure for releasing DNA from bacterial cells.

Applied and environmental microbiology ·Vol. 51 ·No. 5 ·1986-05-00 ·Pages 1138-40

Heath LS, Sloan GL, Heath HE

Abstract

Treatment of Staphylococcus simulans biovar staphylolyticus cells with acetone before digestion with lysozyme made the cells susceptible to lysis by sodium dodecyl sulfate. This technique was found to be useful for releasing DNA from a wide variety of gram-positive and gram-negative organisms.

MeSH Terms
Acetone/pharmacology Bacteriological Techniques DNA, Bacterial Electrophoresis, Agar Gel Muramidase/metabolism Sodium Dodecyl Sulfate/metabolism Staphylococcus/drug effects,genetics
Chemicals
DNA, Bacterial Acetone Sodium Dodecyl Sulfate Muramidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Heath L S
Sloan G L
Heath H E
References (12)
12 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.51.5.1138-1140.1986
Published
1986-05-00
Pages
1138-40
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC239025
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.51.5.1138-1140.1986 PMC Full Text View journal details
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