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PMID: 3693402 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Translocation of acyl-CoA oxidase into peroxisomes requires ATP hydrolysis but not a membrane potential.

The Journal of cell biology ·Vol. 105 ·No. 6 Pt 2 ·1987-12-00 ·Pages 2915-22

Imanaka T, Small GM, Lazarow PB

Abstract

An efficient system for the import of newly synthesized proteins into highly purified rat liver peroxisomes was reconstituted in vitro. 35S-Labeled acyl-CoA oxidase (AOx) was incorporated into peroxisomes in a proteinase K-resistant fashion. This import was specific (did not occur with mitochondria) and was dependent on temperature, time, and peroxisome concentration. Under optimal conditions approximately 30% of [35S]AOx became proteinase resistant. The import of AOx into peroxisomes could be dissociated into two steps: (a) binding occurred at 0 degrees C in the absence of ATP; (b) translocation occurred only at 26 degrees C and required the hydrolysis of ATP. GTP would not substitute for ATP and translocation was not inhibited by carbonylcyanide-m-chlorophenylhydrazone, valinomycin, or other ionophores.

MeSH Terms
Acyl-CoA Oxidase Adenosine Triphosphate/metabolism Biological Transport, Active Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology Cell Fractionation Centrifugation, Density Gradient In Vitro Techniques Iohexol Ionophores/pharmacology Kinetics Magnesium/pharmacology Magnesium Chloride Membrane Potentials Microbodies/metabolism Oxidoreductases/metabolism Potassium Chloride/pharmacology Temperature
Chemicals
Ionophores Magnesium Chloride Iohexol Carbonyl Cyanide m-Chlorophenyl Hydrazone Potassium Chloride Adenosine Triphosphate Oxidoreductases Acyl-CoA Oxidase Magnesium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Imanaka T
Rockefeller University, New York 10021.
Small G M
Lazarow P B
References (40)
40 references, click to expand
  1. ATP is essential for protein translocation into Escherichia coli membrane vesicles.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4384-8 PMID: 2861605
  2. Protein import into chloroplasts requires a chloroplast ATPase.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3288-92 PMID: 2953027
  3. Proton ionophores prevent assembly of a peroxisomal protein.
    Cell. 1987 Jan 16;48(1):165-73 PMID: 3539364
  4. Large cation-selective pores from rat liver peroxisomal membranes incorporated to planar lipid bilayers.
    J Membr Biol. 1986;94(3):285-91 PMID: 2435912
  5. Synthesis of 3-ketoacyl-CoA thiolase of rat liver peroxisomes on free polyribosomes as a larger precursor. Induction of thiolase mRNA activity by clofibrate.
    Biochem J. 1985 Mar 15;226(3):697-704 PMID: 3985942
  6. Transport of F1-ATPase subunit beta into mitochondria depends on both a membrane potential and nucleoside triphosphates.
    FEBS Lett. 1986 Dec 15;209(2):152-6 PMID: 2878825
  7. Biogenesis of peroxisomes.
    Annu Rev Cell Biol. 1985;1:489-530 PMID: 3916321
  8. Permeability of the peroxisomal membrane to cofactors of beta-oxidation. Evidence for the presence of a pore-forming protein.
    J Biol Chem. 1987 Mar 25;262(9):4310-8 PMID: 3031070
  9. Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.
    J Cell Biol. 1982 Apr;93(1):97-102 PMID: 7068762
  10. The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions.
    J Cell Biol. 1968 May;37(2):482-513 PMID: 4297786
  11. Secretion in yeast: translocation and glycosylation of prepro-alpha-factor in vitro can occur via an ATP-dependent post-translational mechanism.
    EMBO J. 1986 May;5(5):1031-6 PMID: 15957217
  12. Post-translational insertion of a fragment of the glucose transporter into microsomes requires phosphoanhydride bond cleavage.
    Nature. 1986 Aug 7-13;322(6079):549-52 PMID: 3736672
  13. Phosphodiester bond cleavage outside mitochondria is required for the completion of protein import into the mitochondrial matrix.
    Cell. 1987 Jun 5;49(5):651-8 PMID: 3034431
  14. Acyl-Coa oxidase and hydratase-dehydrogenase, two enzymes of the peroxisomal beta-oxidation system, are synthesized on free polysomes of clofibrate-treated rat liver.
    J Cell Biol. 1984 Dec;99(6):2241-6 PMID: 6501422
  15. Both ATP and an energized inner membrane are required to import a purified precursor protein into mitochondria.
    EMBO J. 1987 Apr;6(4):1073-7 PMID: 3036490
  16. Peroxisomes (microbodies and related particles).
    Physiol Rev. 1966 Apr;46(2):323-57 PMID: 5325972
  17. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  18. Hepatic and renal effects of peroxisome proliferators: biological implications.
    Ann N Y Acad Sci. 1982;386:81-110 PMID: 7046570
  19. Post-translational import of fatty acyl-CoA oxidase and catalase into peroxisomes of rat liver in vitro.
    J Biol Chem. 1985 May 10;260(9):5603-9 PMID: 4039322
  20. Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis.
    J Cell Biol. 1986 May;102(5):1543-50 PMID: 3517001
  21. Distinct steps in the import of ADP/ATP carrier into mitochondria.
    J Biol Chem. 1987 Jun 5;262(16):7528-36 PMID: 3034898
  22. Energy dependence of protein translocation into chloroplasts.
    Eur J Biochem. 1986 Nov 3;160(3):563-70 PMID: 3780721
  23. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  24. Cell biology. An unfolding story of protein translocation.
    Nature. 1986 Jul 17-23;322(6076):209-10 PMID: 3526158
  25. Import of the carboxy-terminal portion of acyl-CoA oxidase into peroxisomes of Candida tropicalis.
    J Cell Biol. 1987 Jul;105(1):247-50 PMID: 3611187
  26. Nature of the hepatomegalic effect produced by ethyl-chlorophenoxy-isobutyrate in the rat.
    Nature. 1965 Nov 27;208(5013):856-8 PMID: 5870099
  27. Two fractions of rough endoplasmic reticulum from rat liver. II. Cytoplasmic messenger RNA's which code for albumin and mitochondrial proteins are distributed differently between the two fractions.
    J Cell Biol. 1977 Mar;72(3):726-43 PMID: 838773
  28. Identification of beta-oxidation enzymes among peroxisomal polypeptides. Increase in Coomassie blue-stainable protein after clofibrate treatment.
    FEBS Lett. 1982 Dec 27;150(2):307-10 PMID: 7160475
  29. Transcription regulation of peroxisomal fatty acyl-CoA oxidase and enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase in rat liver by peroxisome proliferators.
    Proc Natl Acad Sci U S A. 1986 Mar;83(6):1747-51 PMID: 3456610
  30. Both ATP and the electrochemical potential are required for optimal assembly of pro-OmpA into Escherichia coli inner membrane vesicles.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4219-22 PMID: 2872675
  31. In vitro translocation of protein across Escherichia coli membrane vesicles requires both the proton motive force and ATP.
    J Biol Chem. 1987 Feb 15;262(5):2358-62 PMID: 3029075
  32. Protein phosphorylation in peroxisomes.
    Biochem Biophys Res Commun. 1986 Oct 15;140(1):188-94 PMID: 3778443
  33. Import of an incompletely folded precursor protein into isolated mitochondria requires an energized inner membrane, but no added ATP.
    EMBO J. 1987 Aug;6(8):2449-56 PMID: 2822394
  34. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  35. Partial disassembly of peroxisomes.
    J Cell Biol. 1985 Jul;101(1):294-304 PMID: 2989301
  36. Water- and solute-accessible spaces of purified peroxisomes. Evidence that peroxisomes are permeable to NAD+.
    Biochem J. 1983 Mar 15;210(3):685-93 PMID: 6870803
  37. Inhibition of glycosidases by aldonolactones of corresponding configuration. 2. Inhibitors of beta-N-acetylglucosaminidase.
    Biochem J. 1958 Jul;69(3):467-76 PMID: 13560392
  38. In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent posttranslational translocation of the prepro-alpha-factor.
    Cell. 1986 May 9;45(3):397-406 PMID: 3009026
  39. A fatty acyl-CoA oxidizing system in rat liver peroxisomes; enhancement by clofibrate, a hypolipidemic drug.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):2043-6 PMID: 180535
  40. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.105.6.2915
Published
1987-12-00
Pages
2915-22
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114735
Subset
IM
Grants
NIADDK NIH HHS · AM 19394 · United States
External Links
PubMed source DOI: 10.1083/jcb.105.6.2915 PMC Full Text View journal details
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