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PMID: 3710562 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Influence of plasma proteins on activity of proinflammatory enzyme phospholipase A2.

Inflammation ·Vol. 10 ·No. 2 ·1986-06-00 ·Pages 183-93

Vadas P, Stefanski E, Pruzanski W

Abstract

The influence of plasma proteins on quantitation of the proinflammatory enzyme phospholipase A2 (PLA2) from rheumatoid synovial fluid was studied using two different assays. Human and bovine serum albumins increased the rate of PLA2 hydrolysis of membrane-associated phospholipid substrates. In contrast, albumin profoundly inhibited the PLA2 hydrolysis of synthetic phospholipids in micellar dispersion. Other plasma proteins (alpha, beta and gamma-globulins) had minimal effect on PLA2 activity in either assay system. Since the presence of albumin may compromise estimation of PLA2 and of phospholipase-inhibitory proteins, the appropriate selection of assay conditions is obligatory for the accurate quantitation of their respective activities.

MeSH Terms
Arthritis, Rheumatoid/enzymology,pathology Blood Proteins/pharmacology Humans Inflammation Phospholipases/metabolism Phospholipases A/antagonists & inhibitors,metabolism Phospholipases A2 Serum Albumin/pharmacology Serum Globulins/pharmacology Synovial Fluid/enzymology
Chemicals
Blood Proteins Serum Albumin Serum Globulins Phospholipases Phospholipases A Phospholipases A2
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vadas P
Stefanski E
Pruzanski W
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39 references, click to expand
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Article Info
Journal
Inflammation
Abbr.
Inflammation
ISSN
0360-3997
DOI
10.1007/BF00916000
Published
1986-06-00
Pages
183-93
Language
English
Region
United States
NLM ID
7600105
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00916000 View journal details
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