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PMID: 374378 Published · ppublish English Journal Article

Clustering of genes involved in replication, copy number control, incompatibility, and stable maintenance of the resistance plasmid R1drd-19.

Journal of bacteriology ·Vol. 138 ·No. 1 ·1979-04-00 ·Pages 70-9

Molin S, Stougaard P, Uhlin BE, Gustafsson P, Nordström K

Abstract

Plasmid R1drd-19 is present in a small number of copies per cell of Escherichia coli. The plasmid was reduced in size by in vivo as well as in vitro (cloning) techniques, resulting in a series of plasmid derivatives of different molecular weight. All plasmids isolated contain a small region (about 2 x 10(6) daltons of deoxyribonucleic acid) of the resistance transfer factor part of the plasmid located close to one of the IS1 sequences that separates the resistance transfer factor part from the resistance determinant. All these derivatives were present at the same copy number, retained the incompatibility properties of plasmid R1drd-19, and were stably maintained during cell division. Genes mutated to yield copy mutations also were found to be located in the same region.

MeSH Terms
Cell Division DNA Replication DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/genetics,growth & development Genes Mutation R Factors
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Molin S
Stougaard P
Uhlin B E
Gustafsson P
Nordström K
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34 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.138.1.70-79.1979
Published
1979-04-00
Pages
70-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218239
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.138.1.70-79.1979 PMC Full Text View journal details
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