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PMID: 3774543 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterisation of chicken erythroid nuclear proteins which bind to the nuclease hypersensitive regions upstream of the beta A- and beta H-globin genes.

Nucleic acids research ·Vol. 14 ·No. 19 ·1986-10-10 ·Pages 7675-93

Plumb MA, Lobanenkov VV, Nicolas RH, Wright CA, Zavou S, Goodwin GH

Abstract

Chicken erythrocyte sequence-specific nuclear DNA-binding proteins, which bind to the 5'-flanking DNAseI hypersensitive sites of the erythrocyte chromosomal beta A- and beta H-globin genes, have been fractionated by HPLC gel filtration. Three beta A-globin gene DNA binding activities (to sites A, B and B' (10-12)) were separated. The erythroid precursor cell line HD3 has beta A-globin gene sites B and B' binding activities, but binding to site A is detected only after the HD3 cells are induced to differentiate. The fractionated protein binds to a redefined site B', which contains at its center the globin CACCC consensus sequence. The chromosomal beta H-globin gene has two 5'-flanking DNAseI hypersensitive sites which bracket two sequences (H and H') bound by erythrocyte and HD3 nuclear protein in vitro. The beta H- and beta A-globin gene binding sites (H and B) contain variants of the sequences bound by Nuclear Factor 1 and the TGGCA-binding protein, and their protein binding activity(ies) co-purify after HPLC gel filtration.

MeSH Terms
Animals Base Sequence Binding Sites Chickens Chromatography, Gel Chromatography, High Pressure Liquid DNA-Binding Proteins/analysis,isolation & purification Deoxyribonuclease I/pharmacology Erythrocytes/analysis Gene Expression Regulation Globins/genetics Molecular Weight Nucleoproteins/analysis,isolation & purification
Chemicals
DNA-Binding Proteins Nucleoproteins Globins Deoxyribonuclease I
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Plumb M A
Lobanenkov V V
Nicolas R H
Wright C A
Zavou S
Goodwin G H
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.19.7675
Published
1986-10-10
Pages
7675-93
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311788
Subset
IM
Databases
GENBANK
X13142
External Links
PubMed source DOI: 10.1093/nar/14.19.7675 PMC Full Text View journal details
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