Home LiteratureArticle Details

PMID: 3789719 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Cadmium- and mercury-resistant Bacillus strains from a salt marsh and from Boston Harbor.

Applied and environmental microbiology ·Vol. 52 ·No. 6 ·1986-12-00 ·Pages 1293-8

Mahler I, Levinson HS, Wang Y, Halvorson HO

Abstract

Bacteria resistant to cadmium or mercury or both were isolated from the Great Sippewissett Marsh (Cape Cod, Mass.) and from Boston Harbor. Many of these metal-resistant isolates were gram-positive aerobic sporeformers, although not necessarily isolated as spores. Although several of the isolated strains bore plasmids, cadmium and mercury resistances appeared to be, for the most part, chromosomally encoded. DNA sequence homology of the gram-positive cadmium- and mercury-resistant isolates was not demonstrable with metal resistance genes from plasmids of either gram-positive (pI258) or gram-negative (pDB7) origin. Cadmium resistance of all the marsh isolates tested resulted from reduced Cd2+ transport. On the other hand, three cadmium-resistant harbor isolates displayed considerable influx but no efflux of Cd2+. Hg-resistant strains detoxified mercury by transforming Hg2+ to volatile Hg0 via mercuric reductase.

MeSH Terms

Bacillus/drug effects,isolation & purification,metabolism Biological Transport Cadmium/metabolism,pharmacology Drug Resistance, Microbial Manganese/pharmacology Massachusetts Mercury/metabolism,pharmacology Oxidoreductases/metabolism R Factors Seawater Water Microbiology Water Pollution, Chemical

Chemicals

Cadmium Manganese Oxidoreductases mercuric reductase Mercury

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Mahler I

Levinson H S

Wang Y

Halvorson H O

References (26)

26 references, click to expand

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
Enzymatic reduction of mercurous and mercuric ions in Bacillus cereus.
Can J Microbiol. 1981 Feb;27(2):192-7 PMID: 6452192
Cadmium-resistant mutant of Bacillus subtilis 168 with reduced cadmium transport.
J Bacteriol. 1985 Jun;162(3):1106-10 PMID: 3922941
Plasmid-determined resistance to antimicrobial drugs and toxic metal ions in bacteria.
Microbiol Rev. 1983 Sep;47(3):361-409 PMID: 6355806
Biochemical effects of mercury, cadmium, and lead.
Annu Rev Biochem. 1972;41(10):91-128 PMID: 4570963
Cadmium-binding component in Escherichia coli during accommodation to low levels of this ion.
Appl Environ Microbiol. 1981 Jan;41(1):46-50 PMID: 7013701
Mechanisms of microbial resistance and detoxification of mercury and organomercury compounds: physiological, biochemical, and genetic analyses.
Microbiol Rev. 1984 Jun;48(2):95-124 PMID: 6377034
Transformation of Escherichia coli and Bacillus subtilis with a hybrid plasmid molecule.
J Bacteriol. 1977 Jul;131(1):374-7 PMID: 406259
Cadmium and manganese transport in Staphylococcus aureus membrane vesicles.
J Bacteriol. 1982 May;150(2):973-6 PMID: 7068540
Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis.
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1680-2 PMID: 404641
Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis.
J Bacteriol. 1978 Feb;133(2):897-903 PMID: 415051
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Construction of a recombinant plasmid composed of B. subtilis leucine genes and a B. subtilis (natto) plasmid: its use as cloning vehicle in B. subtilis 168.
Mol Gen Genet. 1978 Oct 24;165(3):269-76 PMID: 105237
Development of competence in the Bacillus subtilis transformation system.
J Bacteriol. 1967 Sep;94(3):562-70 PMID: 4962301
Rapid procedure for detection and isolation of large and small plasmids.
J Bacteriol. 1981 Mar;145(3):1365-73 PMID: 7009583
Energy-dependent efflux of cadmium coded by a plasmid resistance determinant in Staphylococcus aureus.
J Bacteriol. 1981 Aug;147(2):313-9 PMID: 7263609
Investigation of cadmium resistance in an Alcaligenes sp.
Appl Environ Microbiol. 1986 Mar;51(3):515-20 PMID: 3963810
Preparation of a DNA gene probe for detection of mercury resistance genes in gram-negative bacterial communities.
Appl Environ Microbiol. 1985 Mar;49(3):686-92 PMID: 3994373
Manganese acquisition by Lactobacillus plantarum.
J Bacteriol. 1984 Apr;158(1):1-8 PMID: 6715278
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
The DNA sequence of the mercury resistance operon of the IncFII plasmid NR1.
J Mol Appl Genet. 1984;2(6):601-19 PMID: 6530603
High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.
Mol Gen Genet. 1979 Jan 5;168(1):111-5 PMID: 107388
Cadmium-Resistant Pseudomonas putida Synthesizes Novel Cadmium Proteins.
Science. 1984 Sep 7;225(4666):1043-6 PMID: 17783048
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
Resistance to mercury and to cadmium in chromosomally resistant Staphylococcus aureus.
Antimicrob Agents Chemother. 1986 Apr;29(4):663-9 PMID: 3635384
Factors affecting transformation of Bacillus licheniformis.
J Bacteriol. 1966 Mar;91(3):1012-20 PMID: 5929742

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.52.6.1293-1298.1986

Published

1986-12-00

Pages

1293-8

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC239224

Subset

External Links

PubMed source DOI: 10.1128/aem.52.6.1293-1298.1986 PMC Full Text View journal details

Analysis Services

Cadmium- and mercury-resistant Bacillus strains from a salt marsh and from Boston Harbor.

Contact

WeChat Official Account