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PMID: 3872187 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two monoclonal antibodies raised against a Burkitt lymphoma cell line recognise different cell types within lymphoid follicles.

Clinical and experimental immunology ·Vol. 59 ·No. 2 ·1985-02-00 ·Pages 315-26

Murray LJ, Dhut S, Walker LC, Rainey M, Habeshaw JA

Abstract

Monoclonal antibodies were raised against a laboratory derived variant of the Raji Burkitt lymphoma cell line. We have characterized two of these antibodies by screening on haematopoietic cell lines, and frozen sections of both reactive and neoplastic lymphoid tissue, and found reactivity with separate cellular components of lymphoid follicles. Monoclonal FW37.4.D5 reacts in section specifically with follicular dendritic reticular cells. Monoclonal 35.1C5 reacts with a subpopulation of splenic marginal zone, and lymph node mantle zone, B lymphocytes, but stains only rare cells within germinal centres. This monoclonal is operationally B lymphocyte specific, distinguishing an 'intermediate' B cell subset, represented in lymphoma by B chronic lymphocytic leukaemia, B hairy cell leukaemia and centrocytic lymphoma.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antibody Specificity B-Lymphocytes/immunology Burkitt Lymphoma/immunology Cell Line Leukemia, Hairy Cell/immunology Leukemia, Lymphoid/immunology Lymphocyte Activation Lymphoid Tissue/immunology Lymphoma/immunology Male Mice Mice, Inbred BALB C Phenotype
Chemicals
Antibodies, Monoclonal
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Murray L J
Dhut S
Walker L C
Rainey M
Habeshaw J A
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Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
Published
1985-02-00
Pages
315-26
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1577118
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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