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PMID: 3898020 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequences of two serine tRNAs with a GGA anticodon: the structure-function relationships in the serine family of E. coli tRNAs.

Nucleic acids research ·Vol. 13 ·No. 15 ·1985-08-12 ·Pages 5697-706

Grosjean H, Nicoghosian K, Haumont E, Söll D, Cedergren R

Abstract

We have determined the nucleotide sequence of the major species of E. coli tRNASer and of a minor species having the same GGA anticodon. These two tRNAs should recognize the UCC and UCU codons, the most widely used codons for serine in the highly expressed genes of E. coli. The two sequences differ in only one position of the D-loop. Neither tRNA has a modified adenosine in the position 3'-adjacent to the anticodon. This can be rationalized on the basis of a structural constraint in the anticodon stem and may be related to optimization of the codon-anticodon interaction. Comparison of all E.coli serine tRNAs (and that encoded by bacteriophage T4) reveals characteristic (possibly functional) features. Evolutionary analysis suggests an eubacterial origin of the T4 tRNASer gene and the existence of a recent common ancestor for the tRNASerGGA and tRNASerGUC genes.

MeSH Terms
Anticodon Base Composition Base Sequence Biological Evolution Codon Escherichia coli/genetics RNA, Transfer RNA, Transfer, Amino Acyl/genetics,metabolism Serine-tRNA Ligase/metabolism Structure-Activity Relationship
Chemicals
Anticodon Codon RNA, Transfer, Amino Acyl RNA, Transfer Serine-tRNA Ligase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Grosjean H
Nicoghosian K
Haumont E
Söll D
Cedergren R
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.15.5697
Published
1985-08-12
Pages
5697-706
Language
English
Region
England
NLM ID
0411011
PMCID
PMC321899
Subset
IM
Databases
GENBANK
K03319, X02802
External Links
PubMed source DOI: 10.1093/nar/13.15.5697 PMC Full Text View journal details
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