Home LiteratureArticle Details
PMID: 392231 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A cold-sensitive mutant of Saccharomyces cerevisiae defective in ribosome processing.

Molecular & general genetics : MGG ·Vol. 175 ·No. 3 ·1979-10-01 ·Pages 313-23

Ursic D, Davies J

Abstract

Cold-sensitive mutants of Saccharomyces cerevisiae isolated by tritium suicide were screened for defects in ribosome biosynthesis. The biochemical defects of mutant dip-1 (defective in processing) were characterized; it is defective in ribosome biosynthesis at the level of production of the primary 35S transcript. At restrictive conditions mutant dip-1 accumulates abnormal rRNA in addition to wild-type rRNA. In the mutant the first observable transcription product was a 14SRNA species which had sequence homologies to 18S rDNA and was the major rRNA component of the 40S ribosomal subunit. In addition, the ribonucleoprotein particles of dip-1 harbored RNA molecules with homologies to yeast rDNA which comprises the spacer region between 18S and 25S rDNA cistrons. Possible causes for the defective production of rRNA and its assembly into subunits are discussed.

MeSH Terms
Cold Temperature DNA, Fungal/genetics Mutation Nucleic Acid Hybridization Phenotype RNA, Ribosomal/genetics Ribosomes/metabolism Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
DNA, Fungal RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ursic D
Davies J
References (30)
30 references, click to expand
  1. Selection of ribosomal mutants by antibiotic suppression in yeast.
    Science. 1971 Dec 24;174(4016):1339-41 PMID: 4944401
  2. Mutation in Saccharomyces cerevisiae conferring streptomycin and cold sensitivity by affecting ribosome formation and function.
    J Bacteriol. 1974 May;118(2):319-28 PMID: 4133351
  3. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  4. Enrichment for temperature-sensitive and auxotrophic mutants in Saccharomyces cerevisiae by tritium suicide.
    Mutat Res. 1973 Mar;17(3):315-22 PMID: 4569495
  5. Isolation of bacteriophage lambda containing yeast ribosomal RNA genes: screening by in situ RNA hybridization to plaques.
    Cell. 1976 Jun;8(2):227-32 PMID: 786462
  6. The genetic control of sporulation in Saccharomyces. I. The isolation of temperature-sensitive sporulation-deficient mutants.
    Genetics. 1969 Jan;61(1):79-89 PMID: 5802566
  7. Restriction endonuclease analysis of ribosomal DNA from Saccharomyces cerevisiae.
    Mol Gen Genet. 1976 Nov 17;148(3):233-41 PMID: 796660
  8. Macromolecule synthesis in yeast spheroplasts.
    J Bacteriol. 1967 Nov;94(5):1697-705 PMID: 6066050
  9. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
    Biochemistry. 1968 Feb;7(2):668-74 PMID: 4868544
  10. Processing of 45 s nucleolar RNA.
    J Mol Biol. 1970 Jan 28;47(2):169-78 PMID: 4314037
  11. Construction and restriction endonuclease mapping of hybrid plasmids containing Saccharomyces cerevisiae ribosomal DNA.
    Mol Gen Genet. 1977 Mar 16;151(3):229-44 PMID: 325373
  12. Ribosomal RNA synthesis in Saccharomyces cerevisiae.
    J Mol Biol. 1972 Mar 28;65(2):227-42 PMID: 4557192
  13. Neurospora crassa cytoplasmic ribosomes: cold-sensitive mutant defective in ribosomal ribonucleic acid synthesis.
    J Bacteriol. 1976 Mar;125(3):1112-9 PMID: 130375
  14. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  15. Identification of ten genes that control ribosome formation in yeast.
    Mol Gen Genet. 1970;109(1):42-56 PMID: 5488085
  16. A temperature-sensitive mutation affecting the mammalian 60 S ribosome.
    J Biol Chem. 1975 Nov 25;250(22):8618-23 PMID: 1184581
  17. Structure and function of E. coli ribosomes. 8. Cold-sensitive mutants defective in ribosome assembly.
    Proc Natl Acad Sci U S A. 1969 Jun;63(2):384-91 PMID: 4895536
  18. Genetic analysis of mutations affecting growth of Saccharomyces cerevisiae at low temperature.
    Genetics. 1974 Aug;77(4):651-9 PMID: 4371644
  19. A yeast mutant defective in the processing of 27S r-RNA precursor.
    Mol Gen Genet. 1976 Feb 27;144(1):29-37 PMID: 772413
  20. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid.
    J Biol Chem. 1973 Jun 10;248(11):3860-75 PMID: 4575197
  21. Histone genes of the sea urchin (S. purpuratus) cloned in E coli: order, polarity, and strandedness of the five histone-coding and spacer regions.
    Cell. 1976 Sep;9(1):147-61 PMID: 788917
  22. Cold-sensitive mutants in Aspergillus nidulans. II. Mutations affecting ribosome production.
    Mol Gen Genet. 1974;134(2):115-32 PMID: 4617158
  23. Low temperature and chloramphenicol induction of respiratory deficiency in a cold-sensitive mutant of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1970 Sep;67(1):52-8 PMID: 5272328
  24. In vivo and in vitro phosphorylation of ribosomal proteins by protein kinases from Saccharomyces cerevisiae.
    Biochemistry. 1976 Jun 1;15(11):2289-96 PMID: 179565
  25. Small ribonucleic acid molecules produced during ribosome biosynthesis in Saccharomyces cerevisiae.
    J Biol Chem. 1975 Mar 25;250(6):2003-7 PMID: 1090615
  26. Characterization of yeast ribosomal DNA fragments generated by EcoR1 restriction endonuclease.
    Mol Gen Genet. 1976 Aug 19;147(2):153-68 PMID: 787755
  27. Cold-sensitive mutants in Aspergillus nidulans. I. Isolation and general characterisation.
    Mol Gen Genet. 1974;134(2):99-113 PMID: 4617164
  28. Maturation of ribosomal ribonucleic acids and the biogenesis of ribosomes.
    Prog Biophys Mol Biol. 1976;31(2):95-144 PMID: 790469
  29. A temperature-sensitive mutation affecting 28S ribosomal RNA production in mammalian cells.
    Proc Natl Acad Sci U S A. 1973 Apr;70(4):1273-7 PMID: 4515626
  30. Genetic analysis of cold-sensitive ribosome maturation mutants of Escherichia coli.
    J Bacteriol. 1974 Mar;117(3):1082-92 PMID: 4591943
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00397231
Published
1979-10-01
Pages
313-23
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00397231
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2