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PMID: 3932064 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes.

The EMBO journal ·Vol. 4 ·No. 10 ·1985-10-00 ·Pages 2539-45

Johnson P, Gagnon J, Barclay AN, Williams AF

Abstract

The MRC OX-8 antigen is a marker of the rat cytotoxic T lymphocytes that consists of disulphide-linked chains of mol. wts. 37 and 32 kd. It is thought to be equivalent to the human T8 and mouse Lyt2,3 antigens (all now called CD8 antigens). MRC OX-8 antigen was purified from thymocytes using a monoclonal antibody column and because antigenicity was retained after reduction and alkylation the two polypeptide chains could be separated by a subsequent affinity chromatography step. Peptides were isolated from each chain and their sequences determined. A cDNA probe coding for the mouse CD8 antigen (pLY2C-1 provided by Dr L. A. Herzenberg) was used to obtain rat cDNA clones from which the sequence of the equivalent rat molecule was determined. Peptides from the 32-kd chain were identified in this translated sequence whereas peptides from the 37-kd chain were not. The 32-kd polypeptide sequence consisted of 210 amino acids and had one possible N-linked glycosylation site. The N-terminal part of the sequence was surprisingly different from both its mouse and human counterparts but, as in the other two species, it showed a clear relationship to Ig V domains.

MeSH Terms
Amino Acid Sequence Animals Antigens, Differentiation, T-Lymphocyte Antigens, Surface/genetics,isolation & purification Base Sequence Cloning, Molecular DNA/genetics Macromolecular Substances Membrane Proteins/genetics,immunology,isolation & purification Molecular Weight Protein Sorting Signals RNA, Messenger/genetics Rats T-Lymphocytes, Cytotoxic/analysis
Chemicals
Antigens, Differentiation, T-Lymphocyte Antigens, Surface Macromolecular Substances Membrane Proteins Protein Sorting Signals RNA, Messenger DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Johnson P
Gagnon J
Barclay A N
Williams A F
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46 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-10-00
Pages
2539-45
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554541
Subset
IM
Databases
GENBANK
X03015
External Links
PubMed source PMC Full Text View journal details
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