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PMID: 3951993 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The beta-globin domain in immature chicken erythrocytes: enhanced solubility is coincident with histone hyperacetylation.

Nucleic acids research ·Vol. 14 ·No. 4 ·1986-02-25 ·Pages 1667-82

Nelson DA, Ferris RC, Zhang DE, Ferenz CR

Abstract

A 60 minute exposure of chicken immature erythrocytes to n-butyrate shifts actively acetylated and deacetylated histones to hypermodified forms. Micrococcal nuclease digestion of nuclei from n-butyrate treated cells and subsequent fractionation of the chromatin releases 40-45% of the adult beta-globin (beta A) nucleohistone into a soluble fraction. This is an eleven fold enrichment over the soluble chromatin from untreated cells (Ferenz and Nelson (1985) Nucleic Acids Res. 13, 1977-1995). The enhanced beta A chromatin solubility and induced histone hyperacetylation are coincident. Removal of n-butyrate from the cell incubation medium allows rapid histone deacetylation and a striking reduction in beta A chromatin solubility. Chromatin from cells incubated in the absence of n-butyrate, or in medium containing 10 mM NaCl or 2% dimethylsulfoxide, does not exhibit histone hyperacetylation, or the acquired solubility of beta A chromatin. We show that the H4 histone co-isolated with the beta A DNA is in a hyperacetylated state and present evidence that the n-butyrate incubation increases the solubility of both coding and noncoding chromatin regions in the beta-globin domain.

MeSH Terms
Acetylation Animals Butyrates/pharmacology Cell Differentiation Chickens Chromatin/physiology Erythrocytes/cytology,physiology Gene Expression Regulation/drug effects Globins/genetics Histones/metabolism Kinetics Solubility
Chemicals
Butyrates Chromatin Histones Globins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nelson D A
Ferris R C
Zhang D E
Ferenz C R
References (64)
64 references, click to expand
  1. Butyric acid, a potent inducer of erythroid differentiation in cultured erythroleukemic cells.
    Cell. 1975 Jul;5(3):319-22 PMID: 1056809
  2. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  3. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  4. Selective synthesis and modification of nuclear proteins during maturation of avian erythroid cells.
    Arch Biochem Biophys. 1976 May;174(1):273-90 PMID: 180895
  5. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
  6. Steroid receptors: elements for modulation of eukaryotic transcription.
    Annu Rev Biochem. 1976;45:721-46 PMID: 183602
  7. n-Butyrate causes histone modification in HeLa and Friend erythroleukaemia cells.
    Nature. 1977 Aug 4;268(5619):462-4 PMID: 268489
  8. Calf liver nuclear N-acetyltransferases. Purification and properties of two enzymes with both spermidine acetyltransferase and histone acetyltransferase activities.
    J Biol Chem. 1978 Jan 10;253(1):233-7 PMID: 22543
  9. Suppression of histone deacetylation in vivo and in vitro by sodium butyrate.
    J Biol Chem. 1978 May 25;253(10):3364-6 PMID: 649576
  10. Structure of chromatin containing extensively acetylated H3 and H4.
    Cell. 1978 Apr;13(4):691-9 PMID: 657272
  11. Sequence of chicken ovalbumin mRNA.
    Nature. 1978 Jun 29;273(5665):723-8 PMID: 661981
  12. Sodium butyrate inhibits histone deacetylation in cultured cells.
    Cell. 1978 May;14(1):105-13 PMID: 667927
  13. The effect of sodium butyrate on histone modification.
    Cell. 1978 May;14(1):115-21 PMID: 667928
  14. Turnover of histone acetyl groups in cultured cells is inhibited by sodium butyrate.
    FEBS Lett. 1978 Jul 1;91(1):117-20 PMID: 668901
  15. DNA associated with hyperacetylated histone is preferentially digested by DNase I.
    Nucleic Acids Res. 1978 Jun;5(6):1863-76 PMID: 673837
  16. Butyrate suppression of histone deacetylation leads to accumulation of multiacetylated forms of histones H3 and H4 and increased DNase I sensitivity of the associated DNA sequences.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2239-43 PMID: 276864
  17. Erythroleukemic differentiation.
    Annu Rev Biochem. 1978;47:419-48 PMID: 354501
  18. DNAse I preferentially digests chromatin containing hyperacetylated histones.
    Biochem Biophys Res Commun. 1978 Jun 29;82(4):1346-53 PMID: 697799
  19. Cloning of chicken lysozyme structural gene sequences synthesized in vitro.
    Nucleic Acids Res. 1978 Sep;5(9):3275-94 PMID: 568256
  20. A correlation between nucleosome spacer region susceptibility to DNase I and histone acetylation.
    Nucleic Acids Res. 1979 Feb;6(2):561-74 PMID: 424305
  21. Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):630-4 PMID: 284387
  22. Isolation and characterization of the histone variants in chicken erythrocytes.
    Biochemistry. 1979 Sep 4;18(18):3952-60 PMID: 486404
  23. Histone-specific acetyltransferases from calf thymus. Isolation, properties, and substrate specificity of three different enzymes.
    Biochemistry. 1980 Mar 4;19(5):943-51 PMID: 7356970
  24. Interaction of HMG 14 and 17 with actively transcribed genes.
    Cell. 1980 Jan;19(1):289-301 PMID: 6244103
  25. Rat liver nuclear N-acetyltransferases: separation of two enzymes with both histone and spermidine acetyltransferase activity.
    Arch Biochem Biophys. 1980 Aug;203(1):384-9 PMID: 7406506
  26. Modulation of the nucleosome structure by histone acetylation.
    Eur J Biochem. 1980 Sep;110(1):143-52 PMID: 7439155
  27. Extensive purification of histone acetylase A, the major histone N-acetyl transferase activity detected in mammalian cell nuclei.
    J Biol Chem. 1980 Dec 10;255(23):11448-53 PMID: 7440547
  28. The effect of histone hyperacetylation on the nuclease sensitivity and the solubility of chromatin.
    J Biol Chem. 1981 Apr 10;256(7):3313-8 PMID: 6259161
  29. Transformation parameters induced in chick cells by incubation in media of altered NaCl concentration.
    Virology. 1981 Jun;111(2):427-39 PMID: 6264668
  30. Manifold effects of sodium butyrate on nuclear function. Selective and reversible inhibition of phosphorylation of histones H1 and H2A and impaired methylation of lysine and arginine residues in nuclear protein fractions.
    J Biol Chem. 1981 Sep 25;256(18):9612-21 PMID: 6270094
  31. Only a small fraction of avian erythrocyte histone is involved in ongoing acetylation.
    Nucleic Acids Res. 1981 Oct 10;9(19):5061-73 PMID: 7312625
  32. Accessibility of newly synthesized chromatin to histone acetylase.
    J Biol Chem. 1982 Apr 10;257(7):3945-9 PMID: 7061519
  33. Silver staining of proteins in polyacrylamide gels.
    Anal Biochem. 1981 Nov 15;118(1):197-203 PMID: 6175245
  34. Histone acetylation increases the solubility of chromatin and occurs sequentially over most of the chromatin. A novel model for the biological role of histone acetylation.
    J Biol Chem. 1982 Jul 10;257(13):7336-47 PMID: 7085629
  35. Chromatin structure of the chicken beta-globin gene region. Sensitivity to DNase I, micrococcal nuclease, and DNase II.
    J Biol Chem. 1982 Jul 10;257(13):7730-6 PMID: 6282852
  36. Hemoglobin switching in chickens. Is the switch initiated post-transcriptionally?
    J Biol Chem. 1982 Sep 25;257(18):11008-14 PMID: 6179944
  37. The chicken beta globin gene region. Delineation of transcription units and developmental regulation of interspersed DNA repeats.
    J Biol Chem. 1982 Sep 25;257(18):11015-23 PMID: 6286679
  38. Studies on histone acetyltransferase. Partial purification and basic properties.
    J Biol Chem. 1982 Nov 10;257(21):12893-900 PMID: 7130185
  39. Hyperacetylation of histone H4 correlates with the terminal, transcriptionally inactive stages of spermatogenesis in rainbow trout.
    Dev Biol. 1982 Oct;93(2):404-15 PMID: 7141104
  40. Acetylation and calcium-dependent phosphorylation of histone H3 in nuclei from butyrate-treated HeLa cells.
    J Biol Chem. 1983 Jan 25;258(2):1299-304 PMID: 6822500
  41. Changes in nucleosomal core histone variants during chicken development and maturation.
    Dev Biol. 1983 Feb;95(2):421-8 PMID: 6825941
  42. Histone H4 hyperacetylation and rapid turnover of its acetyl groups in transcriptionally inactive rooster testis spermatids.
    Nucleic Acids Res. 1982 Dec 20;10(24):8049-59 PMID: 7162988
  43. Histone deacetylase. Association with a nuclease resistant, high molecular weight fraction of HeLa cell chromatin.
    J Biol Chem. 1983 Mar 25;258(6):3726-34 PMID: 6833227
  44. Isolation and partial characterization of three histone-specific acetyltransferases from Artemia.
    Eur J Biochem. 1983 May 2;132(2):249-54 PMID: 6840090
  45. Butyrate-induced histone hyperacetylation in human and mouse cells: estimation of putative sites of histone acetylation in vivo.
    J Cell Biochem. 1982;20(3):225-35 PMID: 7169496
  46. Histone hyperacetylation has little effect on the higher order folding of chromatin.
    Nucleic Acids Res. 1983 Jun 25;11(12):4065-75 PMID: 6866766
  47. Histone deacetylation is required for the maturation of newly replicated chromatin.
    J Biol Chem. 1983 Oct 25;258(20):12675-84 PMID: 6226660
  48. Histone deacetylase from HeLa cells: properties of the high molecular weight complex.
    Biochemistry. 1983 Dec 20;22(26):6175-80 PMID: 6318808
  49. Porcine liver nuclear histone acetyltransferase. Partial purification and basic properties.
    J Biol Chem. 1984 Mar 25;259(6):3413-9 PMID: 6706965
  50. Two species of histone acetyltransferase in rat liver nuclei.
    J Biol Chem. 1984 Jul 10;259(13):8372-7 PMID: 6736037
  51. Hyperacetylation of histone H4 promotes chromatin decondensation prior to histone replacement by protamines during spermatogenesis in rainbow trout.
    Nucleic Acids Res. 1984 Jun 11;12(11):4575-92 PMID: 6739291
  52. Torsional stress promotes the DNAase I sensitivity of active genes.
    Cell. 1984 Dec;39(3 Pt 2):469-78 PMID: 6096006
  53. N-Butyrate incubation of immature chicken erythrocytes preferentially enhances the solubility of beta A chromatin.
    Nucleic Acids Res. 1985 Mar 25;13(6):1977-95 PMID: 4000950
  54. ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS.
    Proc Natl Acad Sci U S A. 1964 May;51:786-94 PMID: 14172992
  55. RNA synthesis and histone acetylation during the course of gene activation in lymphocytes.
    Proc Natl Acad Sci U S A. 1966 Apr;55(4):805-12 PMID: 5219687
  56. High resolution acrylamide gel electrophoresis of histones.
    Arch Biochem Biophys. 1969 Mar;130(1):337-46 PMID: 5778650
  57. Hydrocortisone effect upon the phytohemagglutin-stimulated acetylation of histones in human lymphocytes.
    Biochim Biophys Acta. 1969 Mar 18;179(1):214-20 PMID: 5787645
  58. Acetylation of nuclear protein in the heterochromatin and euchromatin of mealy bugs.
    Exp Cell Res. 1972 Mar;71(1):45-8 PMID: 5025943
  59. A comparative study of histone acetylation, histone deacetylation, and ribonucleic acid synthesis in avian reticulocytes and erythrocytes.
    Biochemistry. 1973 Feb 27;12(5):783-91 PMID: 4568767
  60. Histone acetylation and hormone action. Early effects of oestradiol-17beta on histone acetylation in rat uterus.
    Biochem J. 1972 Dec;130(3):663-9 PMID: 4664926
  61. Histone acetylation and hormone action. Early effects of aldosterone on histone acetylation in rat kidney.
    Biochem J. 1973 Aug;134(4):907-12 PMID: 4762763
  62. Enzymatic modifications and their possible roles in regulating the binding of basic proteins to DNA and in controlling chromosomal structure.
    Cold Spring Harb Symp Quant Biol. 1974;38:803-19 PMID: 4524789
  63. Histones of Drosophila embryos. Electrophoretic isolation and structural studies.
    J Biol Chem. 1974 Jun 25;249(12):3729-36 PMID: 4209264
  64. Partial characterization of a histone acetyltransferase from trout testis.
    Can J Biochem. 1975 Jul;53(7):796-803 PMID: 1156948
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.4.1667
Published
1986-02-25
Pages
1667-82
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339553
Subset
IM
Grants
NIGMS NIH HHS · GM29442 · United States
External Links
PubMed source DOI: 10.1093/nar/14.4.1667 PMC Full Text View journal details
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