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PMID: 4141893 Published · ppublish English Journal Article

Amino acid sequence of the smaller basic protein from rat brain myelin.

The Biochemical journal ·Vol. 141 ·No. 1 ·1974-07-00 ·Pages 243-55

Dunkley PR, Carnegie PR

Abstract

1. The complete amino acid sequence of the smaller basic protein from rat brain myelin was determined. This protein differs from myelin basic proteins of other species in having a deletion of a polypeptide of 40 amino acid residues from the centre of the molecule. 2. A detailed comparison is made of the constant and variable regions in a group of myelin basic proteins from six species. 3. An arginine residue in the rat protein was found to be partially methylated. The ratio of methylated to unmethylated arginine at this position differed from that found for the human basic protein. 4. Three tryptic peptides were isolated in more than one form. The differences between the two forms of each peptide are discussed in relation to the electrophoretic heterogeneity of myelin basic proteins, which is known to occur at alkaline pH values. 5. Detailed evidence for the amino acid sequence of the protein has been deposited as Supplementary Publication SUP 50029 at the British Library (Lending Division) (formerly the National Lending Library for Science and Technology), Boston Spa, Yorks. LS23 7BQ, U.K., from whom copies may be obtained on the terms given in Biochem. J. (1973) 131, 5.

MeSH Terms
Amino Acid Sequence Aminopeptidases Animals Arginine/analysis Brain Chemistry Carboxypeptidases Chromatography Chymotrypsin Electrophoresis, Paper Hydrogen-Ion Concentration Methylation Myelin Basic Protein/analysis Myelin Sheath/analysis Rats Thermolysin
Chemicals
Myelin Basic Protein Arginine Carboxypeptidases Aminopeptidases Chymotrypsin Thermolysin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dunkley P R
Carnegie P R
References (44)
44 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1410243
Published
1974-07-00
Pages
243-55
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1168071
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1410243 PMC Full Text View journal details
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