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PMID: 4152321 Published · ppublish English Comparative Study Journal Article

The undifferentiated and extended forms of C1300 murine neuroblastoma. An ultrastructural study and detection of concanavalin A binding sites on the plasma membrane.

The American journal of pathology ·Vol. 76 ·No. 2 ·1974-08-00 ·Pages 285-312

Graham DI, Gonatas NK, Charalampous FC

Abstract

Mouse neuroblastoma cells (clone neuro-2A) in the undifferentiated and "differentiated" form were compared by light and electron microscopy. "Cytodifferentiation" was induced in monolayer cultures by the addition of dibutyryl-cyclic AMP. The pattern of concanavalin A binding sites was studied after coupling with horseradish peroxidase. The following major differences were observed. The differentiated cells are characterized by numerous and long neurites, aggregation of ribosomes into polysomes, an extensive network of neurofilaments and microtubules, many dense-core neurosecretory-like vesicles, a discontinuous pattern of concanavalin A binding sites on the plasma membrane, and an increase of the specific activities of acetylcholinesterase, choline acetylase and tyrosine hydroxylase. In contrast, the undifferentiated cells grown in suspension culture lack neurites, contain dispersed ribosomes, infrequent neurofilaments and microtubules and dense-core neurosecretory-like vesicles, and exhibit a continuous pattern of concanavalin A binding sites. In addition, the specific activities of the above mentioned enzymes are significantly lower.

MeSH Terms
Acetylcholinesterase/metabolism Acetyltransferases/metabolism Animals Binding Sites Bucladesine/pharmacology Cell Line Cell Membrane Cell Nucleus Cell Transformation, Neoplastic/drug effects Clone Cells Concanavalin A Mice Mice, Inbred A Microscopy, Electron Microtubules Neoplasms, Experimental Neuroblastoma/enzymology,pathology Organoids Peroxidases Polyribosomes Ribosomes Tyrosine 3-Monooxygenase/metabolism
Chemicals
Concanavalin A Bucladesine Peroxidases Tyrosine 3-Monooxygenase Acetyltransferases Acetylcholinesterase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Graham D I
Gonatas N K
Charalampous F C
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1974-08-00
Pages
285-312
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1910860
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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