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PMID: 415308 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Immunoglobulin light chain mRNA is processed from large nuclear RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 1 ·1978-01-00 ·Pages 342-5

Gilmore-Hebert M, Wall R

Abstract

Recombinant DNA probes, produced by the molecular cloning of immunoglobulin kappa light chain mRNA, have been used to analyze heterogenous nuclear RNA for presumptive precursors to cytoplasmic kappa light chain mRNA, Three discrete classes of nuclear RNA containing kappa mRNA sequences were detected after pulse-labeling of immunoglobulin-producing P3 myeloma cells. Two of these were substantially larger than kappa mRNA (approximately 10 and 4 times larger); the third was similar in size to kappa mRNA. Beginning with the largest, the sequential appearance of these three classes of nuclear RNA preceded the first appearance of newly synthesized kappa light chain mRNA in the cytoplasm. The results presented here suggest that immunoglobulin kappa light chain mRNA is generated by the stepwise cleavage and processing of a large nuclear RNA transcript.

MeSH Terms
Cell Line Cell Nucleus/metabolism Cytoplasm/metabolism DNA, Recombinant Immunoglobulin Light Chains/genetics Immunoglobulin kappa-Chains/genetics Molecular Weight Myeloma Proteins Nucleic Acid Precursors/genetics,metabolism RNA, Messenger/genetics,metabolism
Chemicals
DNA, Recombinant Immunoglobulin Light Chains Immunoglobulin kappa-Chains Myeloma Proteins Nucleic Acid Precursors RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gilmore-Hebert M
Wall R
References (29)
29 references, click to expand
  1. Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.
    Nucleic Acids Res. 1975 Dec;2(12):2365-78 PMID: 802513
  2. Globin RNA precursor molecules: biosynthesis and process in erythroid cells.
    Cell. 1977 Jul;11(3):641-50 PMID: 884738
  3. A general method for cloning eukaryotic structural gene sequences.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3146-50 PMID: 61588
  4. Purification of globin messenger RNA from dimethylsulfoxide-induced Friend cells and detection of a putative globin messenger RNA precursor.
    J Mol Biol. 1976 Oct 5;106(4):1067-75 PMID: 824454
  5. A precursor of globin messenger RNA.
    J Mol Biol. 1976 Sep 15;106(2):403-20 PMID: 978727
  6. The relationship between hnRNA and mRNA.
    Prog Nucleic Acid Res Mol Biol. 1976;19:275-92 PMID: 1019347
  7. Amplification and characterization of a beta-globin gene synthesized in vitro.
    Cell. 1976 Jun;8(2):163-82 PMID: 61066
  8. RNA metabolism of murine leukemia virus: size analysis of nuclear pulse-labeled virus-specific RNA.
    Cell. 1977 Jun;11(2):297-305 PMID: 560909
  9. The definition of a large viral transcription unit late in Ad2 infection of HeLa cells: mapping by effects of ultraviolet irradiation.
    Cell. 1977 Apr;10(4):617-21 PMID: 862023
  10. The definition of a large viral transcription unit late in Ad2 infection of HeLa cells: mapping of nascent RNA molecules labeled in isolated nuclei.
    Cell. 1977 Apr;10(4):611-6 PMID: 862022
  11. Intermolecular duplexes in heterogeneous nuclear RNA from HeLa cells.
    Cell. 1977 Apr;10(4):597-610 PMID: 862021
  12. mRNA structure and function.
    Prog Nucleic Acid Res Mol Biol. 1976;19:493-511 PMID: 1019360
  13. Visualization of the silk fibroin transcription unit and nascent silk fibroin molecules on polyribosomes of Bombyx mori.
    Prog Nucleic Acid Res Mol Biol. 1976;19:313-8 PMID: 1019350
  14. Biogenesis of silk fibroin mRNA: an example of very rapid processing?
    Prog Nucleic Acid Res Mol Biol. 1976;19:301-12 PMID: 1019349
  15. Further evidence on the nuclear origin and transfer to the cytoplasm of polyadenylic acid sequences in mammalian cell RNA.
    J Mol Biol. 1973 Apr 15;75(3):515-32 PMID: 4125135
  16. Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA.
    Nature. 1976 Mar 18;260(5548):221-5 PMID: 768776
  17. Kinetics of formation of 5' terminal caps in mRNA.
    Cell. 1976 Jul;8(3):433-42 PMID: 954097
  18. Purification of 14S messenger RNA of immunoglobulin light chain that codes for a possible light-chain precursor.
    Proc Natl Acad Sci U S A. 1973 Feb;70(2):451-5 PMID: 4510289
  19. Purification and properties of biologically active messenger RNA for a myeloma light chain.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1967-71 PMID: 4505674
  20. Ovalbumin messenger RNA: evidence that the initial product of transcription is the same size as polysomal ovalbumin messenger.
    Proc Natl Acad Sci U S A. 1974 Nov;71(11):4327-31 PMID: 4530986
  21. Biogenesis and characterization of SV40 and polyoma RNAs in productively infected cells.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:165-78 PMID: 169056
  22. Relationship of chain transcription to poly(A) addition and processing of hnRNA in HeLa cells.
    Cell. 1974 Nov;3(3):255-64 PMID: 4434473
  23. Production of viral mRNA in adenovirus- transformed cells by the post- transcriptional processing of heterogeneous nuclear RNA containing viral and cell sequences.
    J Virol. 1973 Jun;11(6):953-60 PMID: 4736534
  24. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  25. Methylated, blocked 5 termini in HeLa cell mRNA.
    Proc Natl Acad Sci U S A. 1975 May;72(5):1904-8 PMID: 1057180
  26. Adenovirus-2 mRNA is transcribed as part of a high-molecular-weight precursor RNA.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4445-9 PMID: 1060124
  27. The size of fibroin messenger RNA and its polyadenylic acid content.
    Cell. 1975 Mar;4(3):199-205 PMID: 1122553
  28. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. 3. Assembly of the three subclasses of IgG.
    J Exp Med. 1971 Nov 1;134(5):1316-34 PMID: 5112205
  29. Mouse myelomas and lymphomas in culture.
    Exp Cell Res. 1970 Apr;60(1):61-77 PMID: 5439579
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.1.342
Published
1978-01-00
Pages
342-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411244
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.1.342 PMC Full Text View journal details
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