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PMID: 4595202 Published · ppublish English Journal Article

Biosynthesis of ubiquinone in Escherichia coli K-12: biochemical and genetic characterization of a mutant unable to convert chorismate into 4-hydroxybenzoate.

Journal of bacteriology ·Vol. 118 ·No. 1 ·1974-04-00 ·Pages 41-5

Lawrence J, Cox GB, Gibson F

Abstract

A mutant strain of Escherichia coli unable to carry out the first specific reaction of ubiquinone biosynthesis, that is the conversion of chorismate into 4-hydroxybenzoate, has been isolated. The gene concerned maps at about minute 79 on the E. coli chromosome and has been designated ubiC. This gene is probably the structural gene for chorismate lyase since cell extracts from a transductant strain carrying the ubiC437 mutant allele are unable to convert chorismate into 4-hydroxybenzoate and growing cells of the mutant do not form appreciable quantities of ubiquinone unless 4-hydroxybenzoate is added to the growth medium.

MeSH Terms
Anthranilate Synthase/metabolism Benzoates/biosynthesis,metabolism Cell-Free System Chromosome Mapping Conjugation, Genetic Culture Media Cyclohexanecarboxylic Acids/metabolism Escherichia coli/enzymology,growth & development,metabolism Genes Mutation Succinates/metabolism Transduction, Genetic Ubiquinone/biosynthesis Vinyl Compounds/metabolism
Chemicals
Benzoates Culture Media Cyclohexanecarboxylic Acids Succinates Vinyl Compounds Ubiquinone Anthranilate Synthase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lawrence J
Cox G B
Gibson F
References (24)
24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.118.1.41-45.1974
Published
1974-04-00
Pages
41-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246637
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.118.1.41-45.1974 PMC Full Text View journal details
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