Home LiteratureArticle Details
PMID: 4599625 Published · ppublish English Journal Article

Lambda-chain production in human lymphoblast-mouse fibroblast hybrids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 8 ·1973-08-00 ·Pages 2401-5

Orkin SH, Buchanan PD, Yount WJ, Reisner H, Littlefield JW

Abstract

Mutant human lymphoblast cells deficient in hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) activity were hybridized with thymidine kinase (EC 2.7.1.21)-deficient mouse fibroblasts. Hybrid cells were readily selected, as both parental lines were nonreverting and eliminated by hypoxanthine-amethopterinthymidine medium. Human lambda (lambda) chain was the only immunoglobulin chain produced by the lymphoblast parent, as determined by immunofluorescent techniques. Two independent hybrid clones chosen for detailed study synthesized human lambda chain, and continued to do so after prolonged culture. As in both parental lines, no human immunoglobulin heavy chains, complements C3 or C4, or alpha(1)-antitrypsin, or mouse immunoglobulin chains or complement C5 were detectable in the hybrids. Selection against thymidine kinase-containing hybrid cells with 5-bromodeoxyuridine did not eliminate positive lambda-chain reactivity, suggesting that the kinase and lambda-chain loci are not linked. The continued production of an immunoglobulin chain by human lymphoblast-mouse fibroblast hybrids contrasts with the extinction of other differentiated functions in several hybrid systems, and indicates that gene localization and linkage analysis for human immunoglobulin chains should be feasible with this system.

MeSH Terms
Animals Cell Line Fibroblasts Fluorescent Antibody Technique Genes Humans Hybrid Cells/enzymology,metabolism Immunoassay Immunoglobulin Fragments/analysis,biosynthesis Karyotyping Lymphocytes Mice Pentosyltransferases/biosynthesis Thymidine Kinase/biosynthesis
Chemicals
Immunoglobulin Fragments Pentosyltransferases Thymidine Kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Orkin S H
Buchanan P D
Yount W J
Reisner H
Littlefield J W
References (47)
47 references, click to expand
  1. Chromatographic purification of tetramethylrhodamine-immune globulin conjugates and their use in the cellular localization of rabbit gamma-globulin polypeptide chains.
    J Immunol. 1965 Aug;95(2):230-45 PMID: 4158328
  2. Cytogenetic analysis of human lymphoblasts in continuous culture.
    Exp Cell Res. 1965 Oct;40(1):197-200 PMID: 5320250
  3. Synthesis of collagen and hyaluronic acid by fibroblast hybrids.
    Proc Natl Acad Sci U S A. 1966 Jan;55(1):41-4 PMID: 5220871
  4. Studies of the Vi (gamma-2c) subgroup of gamma-globulin. A relationship between concentration and genetic type among normal individuals.
    J Exp Med. 1967 Jan 1;125(1):177-90 PMID: 4163358
  5. Regulation of pigment synthesis in mammalian cells, as studied by somatic hybridization.
    Proc Natl Acad Sci U S A. 1966 Nov;56(5):1437-40 PMID: 5230304
  6. Detection of genetic antigens utilizing gamma globulins coupled to red blood cells.
    Nature. 1967 Jul 1;215(5096):68-9 PMID: 4167883
  7. Infectious mononucleosis: immunoglobulin synthesis by cell lines.
    J Clin Invest. 1968 Oct;47(10):2391-401 PMID: 4175543
  8. Properties and uses of human-mouse hybrid cell lines.
    Nature. 1968 Dec 21;220(5173):1199-202 PMID: 5725979
  9. The life span of the cultured normal cell: concepts derived from studies of human lymphoblasts.
    J Theor Biol. 1968 Aug;20(2):217-26 PMID: 4883103
  10. Origin of the thymidine kinase induced by polyoma virus in productively infected cells.
    J Virol. 1969 Feb;3(2):140-5 PMID: 4304444
  11. Limited periods of gene expression in immunoglobulin-synthesizing cells.
    Science. 1969 Jun 27;164(3887):1524-5 PMID: 4182344
  12. Immunoglobulin production in synchronized cultures of human hematopoietic cell lines. I. Variation of cellular immunoglobulin level with the generation cycle.
    J Immunol. 1969 Oct;103(4):834-43 PMID: 4186919
  13. Chromosomes of 14 hematopoietic cell lines derived from peripheral blood of persons with and without chromosome anomalies.
    J Natl Cancer Inst. 1969 Nov;43(5):1119-28 PMID: 5353241
  14. HL-A antigens from a continuous lymphoid cell line derived from a normal donor. I. Solubilization and serologic characterization.
    J Immunol. 1970 Mar;104(3):560-5 PMID: 5435786
  15. Biochemically marked lymphocytoid lines: establishment of Lesch-Nyhan cells.
    Science. 1970 Oct 2;170(3953):89-90 PMID: 5452899
  16. Products of lymphoid cells in continuous culture.
    Am J Pathol. 1970 Sep;60(3):483-94 PMID: 5459752
  17. Immunoglobulin spots on the surface of rabbit lymphocytes.
    J Exp Med. 1970 Nov;132(5):1001-18 PMID: 4919141
  18. Imbalances of gamma globulin subgroups and gene defects in patients with primary hypogammaglobulinemia.
    J Clin Invest. 1970 Nov;49(11):1957-66 PMID: 4097659
  19. IgG synthesis in hybrid cells from an antibody-producing mouse myeloma and an L cell substrain.
    Nature. 1970 Dec 12;228(5276):1086-7 PMID: 5483162
  20. Epstein-Barr viral antigen in single cell clones of two human leukocytic lines.
    J Virol. 1970 Nov;6(5):699-701 PMID: 4320703
  21. Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma.
    Science. 1971 Jan 8;171(3966):75-7 PMID: 4099030
  22. Regulation of specific functions of glial cells in somatic hybrids. II. Control of inducibility of glycerol-3-phosphate dehydrogenase.
    Proc Natl Acad Sci U S A. 1970 Dec;67(4):1870-7 PMID: 4321349
  23. Expression of differentiated functions in hepatoma cell hybrids. I. Tyrosine aminotransferase in hepatoma-fibroblast hybrids.
    Proc Natl Acad Sci U S A. 1971 Jan;68(1):127-31 PMID: 4395684
  24. Genes for neuronal properties expressed in neuroblastoma x L cell hybrids.
    Proc Natl Acad Sci U S A. 1971 Jan;68(1):234-9 PMID: 5276295
  25. Immunoglobulin production by human lymphocytoid lines and clones: absence of genic exclusion.
    Science. 1971 Apr 23;172(3981):382-3 PMID: 4323795
  26. Biochemical and cytological evidence for triple hybrid cell line formed from fusion of three different cells.
    Science. 1971 Apr 30;172(3982):470-2 PMID: 5550500
  27. Suppression of immunoglobulin synthesis by cellular hybridization.
    Nat New Biol. 1971 May 19;231(20):87-90 PMID: 4103301
  28. Human thymidine kinase gene locus: assignment to chromosome 17 in a hybrid of man and mouse cells.
    Science. 1971 Jul 16;173(3993):244-5 PMID: 5104177
  29. Immunoglobulin G and free kapa-chain synthesis in different clones of a hybrid cell line.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3045-8 PMID: 5002282
  30. Expression of tyrosine aminotransferase activity in somatic-cell heterokaryons: evidence for negative control of enzyme expression.
    Proc Natl Acad Sci U S A. 1971 Oct;68(10):2589-93 PMID: 4109409
  31. Synthesis of Epstein-Barr virus after activation of the viral genome in a "virus-negative" human lymphoblastoid cell (Raji) made resistant to 5-bromodeoxyuridine (thymidine kinase-virus antigen-immunofluorescence-herpesvirus fingerprints).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):78-82 PMID: 4550511
  32. Regulation of specific functions of glial cells in somatic hybrids. I. Control of S100 protein.
    J Cell Physiol. 1971 Oct;78(2):209-16 PMID: 5156706
  33. Gene dosage dependence of pigment synthesis in melanoma x fibroblast hybrids (hamster cells-mouse fibroblast-DOPA-oxidase-irradiation).
    Proc Natl Acad Sci U S A. 1972 Feb;69(2):330-4 PMID: 4621832
  34. Expression of differentiated functions in hepatoma cell hybrids: induction of mouse albumin production in rat hepatoma-mouse fibroblast hybrids.
    Proc Natl Acad Sci U S A. 1972 Mar;69(3):571-5 PMID: 4335066
  35. Biosynthesis of C4 (fourth component of complement) by hybrids of C4-deficient guinea pig cells and HeLa cells.
    Science. 1972 Jun 2;176(4038):1029-31 PMID: 5033180
  36. Hybridization of Burkitt lymphoblastoid cells.
    Science. 1972 Jun 16;176(4040):1245-7 PMID: 4338252
  37. Chemical mutagenesis at the phosphoribosyltransferase locus in cultured human lymphoblasts.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1244-8 PMID: 4504337
  38. Expression of aryl hydrocarbon hydroxylase induction and suppression of tyrosine aminotransferase induction in somatic-cell hybrids.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2179-83 PMID: 4403306
  39. Autosomal linkage between the Gm and Pi loci in man.
    Ann Hum Genet. 1972 Apr;35(4):393-9 PMID: 5073686
  40. Genetics of somatic mammalian cells: demonstration of a human esterase activator gene linked to the adeB gene.
    Proc Natl Acad Sci U S A. 1972 Nov;69(11):3273-7 PMID: 4508320
  41. Lack of expression of differentiation in mouse teratoma-fibroblast somatic cell hybrids.
    Exp Cell Res. 1973 Jan;76(1):191-9 PMID: 4682647
  42. Linkage analysis in man by somatic cell genetics.
    Nature. 1973 Mar 16;242(5394):165-9 PMID: 4695153
  43. Loss of growth hormone production following hybridization of a functional rat pituitary cell strain with a mouse fibroblast line.
    Exp Cell Res. 1971 Dec;69(2):336-44 PMID: 5170772
  44. Regulation of gene expression in somatic cell hybrids: a review.
    In Vitro. 1971 May-Jun;6(6):411-26 PMID: 4950512
  45. The use of drug-resistant markers to study the hybridization of mouse fibroblasts.
    Exp Cell Res. 1966 Jan;41(1):190-6 PMID: 4952025
  46. Activity of mouse complement.
    J Immunol. 1962 Dec;89:861-7 PMID: 13974902
  47. ISOLATION OF BETA IF-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT.
    J Exp Med. 1965 Aug 1;122:277-98 PMID: 14316946
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.8.2401
Published
1973-08-00
Pages
2401-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433744
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.8.2401 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2