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PMID: 4631836 Published · ppublish English Journal Article

Characterization of a stable L-form of Bacillus subtilis 168.

Journal of bacteriology ·Vol. 113 ·No. 1 ·1973-01-00 ·Pages 486-99

Gilpin RW, Young FE, Chatterjee AN

Abstract

A stable L-form of Bacillus subtilis 168 (sal-1) has been isolated which grows and divides logarithmically in liquid medium with a generation time of 60 min. This mutant does not synthesize cell wall as evidenced by chemical, biochemical, and morphological analyses. Antibiotics which specifically inhibit cell wall biosynthesis do not affect the growth of the L-form. Significant differences exist between the membrane proteins of the bacillary form and the L-form. The relative profile of membrane proteins varies with the salt concentration of the medium in both the L-form and the bacillary form.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacillus subtilis/analysis,cytology,drug effects,growth & development,metabolism Bacterial Proteins/analysis Carbon Isotopes Cell Membrane/analysis Cell Wall/metabolism Culture Media Drug Resistance, Microbial Electrophoresis, Polyacrylamide Gel L Forms/analysis,cytology,drug effects,growth & development,metabolism Microscopy, Electron Microscopy, Phase-Contrast Mutation Sodium Chloride
Chemicals
Anti-Bacterial Agents Bacterial Proteins Carbon Isotopes Culture Media Sodium Chloride
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gilpin R W
Young F E
Chatterjee A N
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34 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.113.1.486-499.1973
Published
1973-01-00
Pages
486-99
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251652
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.113.1.486-499.1973 PMC Full Text View journal details
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